Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Troponin T-FS CRISPR/Cas9 KO Plasmid (m): sc-423462
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Troponin T-FS CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Troponin T-FS genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Troponin T-FS Antibody (H-8): sc-166663
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Troponin T-FS CRISPR/Cas9 KO Plasmid (m) | sc-423462 | 20 µg | $397.00 |
Overview
Tnnt3 encodes fast skeletal muscle troponin T (Troponin T-FS), a core component of the thin filament troponin complex that couples cytosolic Ca²⁺ binding to tropomyosin movement and actin–myosin cross-bridge cycling. By anchoring the troponin complex to tropomyosin, Troponin T-FS helps set contraction kinetics and fiber-type–specific contractile performance during excitation–contraction coupling. Tnnt3 regulation intersects with myofibrillogenesis, sarcomere maintenance, and calcium-dependent signaling that tunes muscle adaptation to activity and load. Dysregulation or altered isoform usage of fast troponin T is relevant to studies of skeletal muscle weakness, myofibrillar disorganization, and related neuromuscular disease mechanisms.
Troponin T-FS CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Tnnt3 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Tnnt3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Tnnt3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Troponin T-FS protein expression.
This CRISPR knockout system enables efficient generation of Tnnt3-deficient cell models for investigation of Troponin T-FS signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Tnnt3 exon(s) critical for Troponin T-FS function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Tnnt3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Troponin T-FS CRISPR/Cas9 KO Plasmid (m) and Troponin T-FS CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Tnnt3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Troponin T-FS HDR Plasmid (m) and Troponin T-FS HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Tnnt3 homology arms to support homology-directed repair at defined Tnnt3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.