Troponin C fast skeletal CRISPR/Cas9 KO Plasmid (m): sc-423432

Overview

Tnnc2 encodes troponin C fast skeletal, a Ca2+-binding regulatory subunit of the troponin complex that controls thin filament activation and cross-bridge cycling in fast-twitch skeletal muscle. By sensing cytosolic Ca2+ transients, TNNC2 coordinates tropomyosin movement on actin to modulate actomyosin ATPase activity and contractile force during excitation–contraction coupling. This protein integrates with calcium handling and sarcomere organization pathways, linking intracellular signaling dynamics to mechanical output in myofibers. Altered troponin complex function and Ca2+ sensitivity are relevant to studies of skeletal muscle performance, myofiber-type physiology, and mechanisms contributing to muscle weakness phenotypes.

Troponin C fast skeletal CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Tnnc2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Tnnc2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Tnnc2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Troponin C fast skeletal protein expression.

This CRISPR knockout system enables efficient generation of Tnnc2-deficient cell models for investigation of Troponin C fast skeletal signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting Tnnc2 exon(s) critical for Troponin C fast skeletal function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple Tnnc2 genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by Troponin C fast skeletal CRISPR/Cas9 KO Plasmid (m) and Troponin C fast skeletal CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Tnnc2 locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by Troponin C fast skeletal HDR Plasmid (m) and Troponin C fast skeletal HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Tnnc2 homology arms to support homology-directed repair at defined Tnnc2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.