Troponin C fast skeletal Antibody (E-7): sc-48347

Troponin C fast skeletal Antibody (E-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects Troponin C fast skeletal (TNNC2) of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-TNNC2 antibody (E-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TNNC2 plays a critical role in muscle contraction by facilitating the interaction between actin and myosin, which are essential for muscle fiber contraction and relaxation. TNNC2 is located in the thin filaments of striated muscle and binds calcium ions, which induces a conformational change that allows myosin to interact with actin, ultimately leading to muscle contraction. TNNC2 in fast skeletal muscle is particularly important as TNNC2 has two calcium-binding sites, allowing for rapid responses to changes in calcium concentration, which is crucial for the quick contractions required in fast-twitch muscle fibers. Understanding the function and dynamics of TNNC2 is vital for studying muscle physiology and pathophysiology, including conditions such as muscular dystrophies and heart diseases.

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Troponin C fast skeletal Antibody (E-7) References:

1. Differential effects of bepridil on functional properties of troponin C in slow and fast skeletal muscles.  |  Kischel, P., et al. 1999. Br J Pharmacol. 128: 767-73. PMID: 10516660
2. Function of the N-terminal calcium-binding sites in cardiac/slow troponin C assessed in fast skeletal muscle fibers.  |  Putkey, JA., et al. 1991. J Biol Chem. 266: 14881-4. PMID: 1869527
3. Activation of fast skeletal muscle troponin as a potential therapeutic approach for treating neuromuscular diseases.  |  Russell, AJ., et al. 2012. Nat Med. 18: 452-5. PMID: 22344294
4. Structure and expression of the murine slow/cardiac troponin C gene.  |  Parmacek, MS. and Leiden, JM. 1989. J Biol Chem. 264: 13217-25. PMID: 2753913
5. cDNA clone and expression analysis of rodent fast and slow skeletal muscle troponin I mRNAs.  |  Koppe, RI., et al. 1989. J Biol Chem. 264: 14327-33. PMID: 2760067
6. Intermolecular And Dynamic Investigation of The Mechanism of Action of Reldesemtiv on Fast Skeletal Muscle Troponin Complex Toward the Treatment of Impaired Muscle Function.  |  Issahaku, AR., et al. 2023. Protein J. 42: 263-275. PMID: 36959428
7. Identification of human metabolites of fast skeletal troponin activators Tirasemtiv and Reldesemtiv for doping control purposes.  |  Euler, L., et al. 2024. Drug Test Anal.. PMID: 39138120
8. A direct regulatory role for troponin T and a dual role for troponin C in the Ca2+ regulation of muscle contraction.  |  Potter, JD., et al. 1995. J Biol Chem. 270: 2557-62. PMID: 7852318
9. Structure and regulation of the mouse cardiac troponin I gene.  |  Ausoni, S., et al. 1994. J Biol Chem. 269: 339-46. PMID: 8276817
10. Actin cytoskeleton. Setting the pace of cell movement.  |  Barkalow, K. and Hartwig, JH. 1995. Curr Biol. 5: 1000-2. PMID: 8542272
11. Myosins: matching functions with motors.  |  Baker, JP. and Titus, MA. 1998. Curr Opin Cell Biol. 10: 80-6. PMID: 9484598
12. A new look at thin filament regulation in vertebrate skeletal muscle.  |  Squire, JM. and Morris, EP. 1998. FASEB J. 12: 761-71. PMID: 9657517

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Troponin C fast skeletal Antibody (E-7) has 8 citations in a variety of scientific publications.

Primary antibodies like Anti-Troponin C fast skeletal Antibody (E-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Troponin C fast skeletal Antibody (E-7) Specifications

Alternate Product Name: TNNC2

Product Clone Name: E-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 51-160

Epitope Target: Troponin C

Epitope Species: human

Product Reactivity/Detection: fast skeletal isoform of Troponin C

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 8 citations in various scientific publications

Available Conjugates/Forms for Troponin C fast skeletal Antibody (E-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin