TRK-T3 Antibody (H-11): sc-515054

TRK-T3 Antibody (H-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects TRK-T3 protein of human origin, TFG protein of mouse origin, and the corresponding rat homolog by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-TRK-T3 antibody (H-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TRK-T3 protein is a product of oncogenic rearrangements of the NTRK1 gene, which encodes the Trk A protein, and these rearrangements are frequently observed in thyroid carcinomas. The fusion of the NTRK1 tyrosine kinase domain with 5′-end sequences of different genes results in TRK-T3 oncoprotein, which consists of 1,412 nucleotides of NTRK1 preceded by 598 nucleotides from TFG (TRK-fused gene), a gene that is ubiquitously expressed and located on chromosome 3. TRK-T3 protein includes a coiled-coil motif within the TFG region, which is crucial for forming protein complexes. This structural feature enhances interactions with other signaling molecules, allowing cytoplasmic TRK-T3 protein to bind to and phosphorylate adaptor proteins such as Shc and SNT1/FRS2. These interactions are vital for linking receptor tyrosine kinases to the mitogen-activated protein kinase pathway, thereby facilitating cellular signaling processes that can lead to tumorigenesis. SHP-1 interacts with and down-regulates TRK-T3, highlighting the importance of post-translational regulation in maintaining cellular homeostasis.

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TRK-T3 Antibody (H-11) References:

1. Biological activity of the thyroid TRK-T3 oncogene requires signalling through Shc.  |  Roccato, E., et al. 2002. Br J Cancer. 87: 645-53. PMID: 12237775
2. Role of TFG sequences outside the coiled-coil domain in TRK-T3 oncogenic activation.  |  Roccato, E., et al. 2003. Oncogene. 22: 807-18. PMID: 12584559
3. The signaling adapters fibroblast growth factor receptor substrate 2 and 3 are activated by the thyroid TRK oncoproteins.  |  Ranzi, V., et al. 2003. Endocrinology. 144: 922-8. PMID: 12586769
4. An in vivo functional genetic screen reveals a role for the TRK-T3 oncogene in tumor progression.  |  Edel, MJ., et al. 2004. Oncogene. 23: 4959-65. PMID: 15077169
5. Analysis of SHP-1-mediated down-regulation of the TRK-T3 oncoprotein identifies Trk-fused gene (TFG) as a novel SHP-1-interacting protein.  |  Roccato, E., et al. 2005. J Biol Chem. 280: 3382-9. PMID: 15557341
6. The DNA rearrangement that generates the TRK-T3 oncogene involves a novel gene on chromosome 3 whose product has a potential coiled-coil domain.  |  Greco, A., et al. 1995. Mol Cell Biol. 15: 6118-27. PMID: 7565764

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TRK-T3 Antibody (H-11) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-TRK-T3 Antibody (H-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TRK-T3 Antibody (H-11) Specifications

Alternate Product Name: TRK-T3

Product Clone Name: H-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-210

Epitope Target: TRK-T3

Epitope Species: human

Product Reactivity/Detection: TRK-T3 of human origin, TFG of mouse origin and the corresponding rat homolog

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for TRK-T3 Antibody (H-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin