TRIM72 Antibody (A-10): sc-514706
- TRIM72 Antibody (A-10) is a mouse monoclonal IgG1 κ TRIM72 antibody, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 114-257 mapping within an internal region of TRIM72 of human origin
- TRIM72 Antibody (A-10) is recommended for detection of TRIM72 of human origin by WB, IP, IF and ELISA
- Anti-TRIM72 Antibody (A-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for TRIM72 Antibody (A-10) for WB applications. These reagents are now offered in bundles with TRIM72 Antibody (A-10) (see ordering information below).
TRIM72 Antibody (A-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects TRIM72 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-TRIM72 antibody (A-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TRIM72, also known as MG53, is a 477 amino acid cytoplasmic vesicle membrane protein that plays a crucial role in muscle cell membrane repair by facilitating the assembly of repair machinery at sites of injury. TRIM72 is essential for the transport of dysferlin, another protein involved in membrane repair, to these injury sites, thereby ensuring effective repair patch formation. TRIM72 is implicated in regulating membrane budding and exocytosis, and may influence the mobility of endocytic vesicles containing the potassium channel KV2.1. TRIM72 exists as a homooligomer and features a unique structure that includes one B box-type zinc finger, one B30.2/SPRY domain, and a RING-type zinc finger, all of which contribute to functional capabilities. TRIM72 is encoded by a gene located on human chromosome 16p11.2 and exists in two alternatively spliced isoforms, highlighting complexity and importance in cellular processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
TRIM72 Antibody (A-10) References:
- MG53 regulates membrane budding and exocytosis in muscle cells. | Cai, C., et al. 2009. J Biol Chem. 284: 3314-3322. PMID: 19029292
- MG53 nucleates assembly of cell membrane repair machinery. | Cai, C., et al. 2009. Nat Cell Biol. 11: 56-64. PMID: 19043407
- Membrane repair defects in muscular dystrophy are linked to altered interaction between MG53, caveolin-3, and dysferlin. | Cai, C., et al. 2009. J Biol Chem. 284: 15894-902. PMID: 19380584
- Mitsugumin 53 (MG53) facilitates vesicle trafficking in striated muscle to contribute to cell membrane repair. | Weisleder, N., et al. 2009. Commun Integr Biol. 2: 225-6. PMID: 19641737
- Crystal structure of PRY-SPRY domain of human TRIM72. | Park, EY., et al. 2010. Proteins. 78: 790-5. PMID: 19967786
- TRIM72 negatively regulates myogenesis via targeting insulin receptor substrate-1. | Lee, CS., et al. 2010. Cell Death Differ. 17: 1254-65. PMID: 20139895
- TRIM72, a novel negative feedback regulator of myogenesis, is transcriptionally activated by the synergism of MyoD (or myogenin) and MEF2. | Jung, SY. and Ko, YG. 2010. Biochem Biophys Res Commun. 396: 238-45. PMID: 20399744
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TRIM72 Antibody (A-10) | sc-514706 | 200 µg/ml | $322.00 | |||
TRIM72 Antibody (A-10): m-IgG Fc BP-HRP Bundle | sc-531198 | 200 µg Ab; 10 µg BP | $361.00 | |||
TRIM72 Antibody (A-10): m-IgGκ BP-HRP Bundle | sc-524819 | 200 µg Ab, 40 µg BP | $361.00 | |||
TRIM72 Antibody (A-10): m-IgG1 BP-HRP Bundle | sc-544430 | 200 µg Ab; 20 µg BP | $361.00 | |||
TRIM72 Antibody (A-10) AC | sc-514706 AC | 500 µg/ml, 25% agarose | $424.00 | |||
TRIM72 Antibody (A-10) HRP | sc-514706 HRP | 200 µg/ml | $322.00 | |||
TRIM72 Antibody (A-10) FITC | sc-514706 FITC | 200 µg/ml | $336.00 | |||
TRIM72 Antibody (A-10) PE | sc-514706 PE | 200 µg/ml | $349.00 | |||
TRIM72 Antibody (A-10) Alexa Fluor® 488 | sc-514706 AF488 | 200 µg/ml | $364.00 | |||
TRIM72 Antibody (A-10) Alexa Fluor® 546 | sc-514706 AF546 | 200 µg/ml | $364.00 | |||
TRIM72 Antibody (A-10) Alexa Fluor® 594 | sc-514706 AF594 | 200 µg/ml | $364.00 | |||
TRIM72 Antibody (A-10) Alexa Fluor® 647 | sc-514706 AF647 | 200 µg/ml | $364.00 | |||
TRIM72 Antibody (A-10) Alexa Fluor® 680 | sc-514706 AF680 | 200 µg/ml | $364.00 | |||
TRIM72 Antibody (A-10) Alexa Fluor® 790 | sc-514706 AF790 | 200 µg/ml | $364.00 |