TRIM5 Antibody (D-6): sc-373864
- TRIM5 Antibody (D-6) is a mouse monoclonal IgG1 κ TRIM5 antibody, cited in 3 publications, provided at 200 µg/ml
- raised against amino acids 166-225 mapping within an internal region of TRIM5 of human origin
- TRIM5 Antibody (D-6) is recommended for detection of TRIM5 of human origin by WB, IP, IF and ELISA
- Anti-TRIM5 Antibody (D-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for TRIM5 Antibody (D-6) for WB applications. These reagents are now offered in bundles with TRIM5 Antibody (D-6) (see ordering information below).
TRIM5 Antibody (D-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects TRIM5 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TRIM5 (D-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TRIM5 protein, consisting of 493 amino acids, is a crucial member of the tripartite motif (TRIM) protein family, which is characterized by three zinc-binding domains: a RING domain, a B-box 2, and a coiled-coil domain. These structural features enable TRIM proteins to engage in homomultimerization, allowing them to localize to specific cellular compartments. Notably, TRIM5 also contains a carboxy-terminal B30.2 (SPRY) domain that is essential for mediating innate intracellular retroviral resistance. This domain′s three variable regions form loops that may serve as a binding surface for retroviruses, highlighting TRIM5′s importance in antiviral defense. The trimerization of isoform alpha of TRIM5 significantly enhances affinity for the retroviral capsid, thereby inhibiting viral infection after virus entry into the host cell. The linker region connecting the coiled-coil and B30.2 domains is critical for this trimerization process, underscoring the intricate relationship between TRIM5′s structure and functional capabilities in combating retroviral threats.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
TRIM5 Antibody (D-6) References:
- The B30.2(SPRY) domain of the retroviral restriction factor TRIM5alpha exhibits lineage-specific length and sequence variation in primates. | Song, B., et al. 2005. J Virol. 79: 6111-21. PMID: 15857996
- The contribution of RING and B-box 2 domains to retroviral restriction mediated by monkey TRIM5alpha. | Javanbakht, H., et al. 2005. J Biol Chem. 280: 26933-40. PMID: 15897199
- Disruption of human TRIM5alpha antiviral activity by nonhuman primate orthologues. | Berthoux, L., et al. 2005. J Virol. 79: 7883-8. PMID: 15919943
- TRIM5alpha selectively binds a restriction-sensitive retroviral capsid. | Sebastian, S. and Luban, J. 2005. Retrovirology. 2: 40. PMID: 15967037
- A specific region of 37 amino acid residues in the SPRY (B30.2) domain of African green monkey TRIM5alpha determines species-specific restriction of simian immunodeficiency virus SIVmac infection. | Nakayama, EE., et al. 2005. J Virol. 79: 8870-7. PMID: 15994780
- Human tripartite motif 5alpha domains responsible for retrovirus restriction activity and specificity. | Perez-Caballero, D., et al. 2005. J Virol. 79: 8969-78. PMID: 15994791
- Characterization of TRIM5alpha trimerization and its contribution to human immunodeficiency virus capsid binding. | Javanbakht, H., et al. 2006. Virology. 353: 234-46. PMID: 16808955
- All three variable regions of the TRIM5alpha B30.2 domain can contribute to the specificity of retrovirus restriction. | Ohkura, S., et al. 2006. J Virol. 80: 8554-65. PMID: 16912305
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TRIM5 Antibody (D-6) | sc-373864 | 200 µg/ml | $322.00 | |||
TRIM5 Antibody (D-6): m-IgG Fc BP-HRP Bundle | sc-529592 | 200 µg Ab; 10 µg BP | $361.00 | |||
TRIM5 Antibody (D-6): m-IgGκ BP-HRP Bundle | sc-522535 | 200 µg Ab, 40 µg BP | $361.00 | |||
TRIM5 Antibody (D-6): m-IgG1 BP-HRP Bundle | sc-543596 | 200 µg Ab; 20 µg BP | $361.00 | |||
TRIM5 Antibody (D-6) AC | sc-373864 AC | 500 µg/ml, 25% agarose | $424.00 | |||
TRIM5 Antibody (D-6) HRP | sc-373864 HRP | 200 µg/ml | $322.00 | |||
TRIM5 Antibody (D-6) FITC | sc-373864 FITC | 200 µg/ml | $336.00 | |||
TRIM5 Antibody (D-6) PE | sc-373864 PE | 200 µg/ml | $349.00 | |||
TRIM5 Antibody (D-6) Alexa Fluor® 488 | sc-373864 AF488 | 200 µg/ml | $364.00 | |||
TRIM5 Antibody (D-6) Alexa Fluor® 546 | sc-373864 AF546 | 200 µg/ml | $364.00 | |||
TRIM5 Antibody (D-6) Alexa Fluor® 594 | sc-373864 AF594 | 200 µg/ml | $364.00 | |||
TRIM5 Antibody (D-6) Alexa Fluor® 647 | sc-373864 AF647 | 200 µg/ml | $364.00 | |||
TRIM5 Antibody (D-6) Alexa Fluor® 680 | sc-373864 AF680 | 200 µg/ml | $364.00 | |||
TRIM5 Antibody (D-6) Alexa Fluor® 790 | sc-373864 AF790 | 200 µg/ml | $364.00 |