TRIM32 Antibody (8H8): sc-135588

TRIM32 Antibody (8H8) is a mouse monoclonal IgG2a kappa light chain antibody that detects TRIM32 protein of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). anti-TRIM32 antibody (8H8) is available as the non-conjugated format. Tripartite motif-containing protein 32 (TRIM32) is a member of the tripartite motif (TRIM) protein family, characterized by a unique domain structure that includes a B-box, a RING-finger, and a coiled-coil motif, along with six C-terminal NHL domains. TRIM32 is predominantly expressed in skeletal muscle, where TRIM32 plays a crucial role as an E3 ubiquitin ligase, facilitating the attachment of ubiquitin to target proteins in collaboration with ubiquitin-conjugating enzymes such as UbcH5a, UbcH5c, and UbcH6. The ubiquitination process is vital for regulating protein degradation, cellular signaling, and various cellular processes, making TRIM32 essential for maintaining muscle integrity and function. Mutations in the TRIM32 gene are linked to two forms of autosomal recessive muscular dystrophy, namely limb girdle muscular dystrophy type 2H (LGMD2H) and sarcotubular myopathy (STM), as well as Bardet-Biedl syndrome (BBS), which is characterized by a range of symptoms including pigmentary retinopathy, polydactyly, and obesity. TRIM32 (8H8) antibody effectively detects this important protein, making TRIM32 (8H8) antibody a valuable tool for research into these associated disorders and the underlying mechanisms of muscle function and pathology.

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TRIM32 Antibody (8H8) References:

1. RING protein Trim32 associated with skin carcinogenesis has anti-apoptotic and E3-ubiquitin ligase properties.  |  Horn, EJ., et al. 2004. Carcinogenesis. 25: 157-67. PMID: 14578165
2. Commonality of TRIM32 mutation in causing sarcotubular myopathy and LGMD2H.  |  Schoser, BG., et al. 2005. Ann Neurol. 57: 591-5. PMID: 15786463
3. Hutterite brothers both affected with two forms of limb girdle muscular dystrophy: LGMD2H and LGMD2I.  |  Frosk, P., et al. 2005. Eur J Hum Genet. 13: 978-82. PMID: 15886712
4. Molecular etiopathogenesis of limb girdle muscular and congenital muscular dystrophies: boundaries and contiguities.  |  Guglieri, M., et al. 2005. Clin Chim Acta. 361: 54-79. PMID: 16002060
5. Trim32 is a ubiquitin ligase mutated in limb girdle muscular dystrophy type 2H that binds to skeletal muscle myosin and ubiquitinates actin.  |  Kudryashova, E., et al. 2005. J Mol Biol. 354: 413-24. PMID: 16243356
6. Homozygosity mapping with SNP arrays identifies TRIM32, an E3 ubiquitin ligase, as a Bardet-Biedl syndrome gene (BBS11).  |  Chiang, AP., et al. 2006. Proc Natl Acad Sci U S A. 103: 6287-92. PMID: 16606853
7. TRIM32 ubiquitin E3 ligase, one enzyme for several pathologies: From muscular dystrophy to tumours.  |  Lazzari, E. and Meroni, G. 2016. Int J Biochem Cell Biol. 79: 469-477. PMID: 27458054
8. TRIM32: A Multifunctional Protein Involved in Muscle Homeostasis, Glucose Metabolism, and Tumorigenesis.  |  Bawa, S., et al. 2021. Biomolecules. 11: PMID: 33802079
9. TRIM32 and Malin in Neurological and Neuromuscular Rare Diseases.  |  Kumarasinghe, L., et al. 2021. Cells. 10: PMID: 33917450
10. TRIM32 promotes oral squamous cell carcinoma progression by enhancing FBP2 ubiquitination and degradation.  |  Yang, X., et al. 2023. Biochem Biophys Res Commun. 678: 165-172. PMID: 37640002
11. CDK2-activated TRIM32 phosphorylation and nuclear translocation promotes radioresistance in triple-negative breast cancer.  |  Tang, J., et al. 2024. J Adv Res. 61: 239-251. PMID: 37734566
12. ATM-CHK2-TRIM32 axis regulates ATG7 ubiquitination to initiate autophagy under oxidative stress.  |  Liu, J., et al. 2023. Cell Rep. 42: 113402. PMID: 37943659

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TRIM32 Antibody (8H8) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-TRIM32 Antibody (8H8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TRIM32 Antibody (8H8) Specifications

Alternate Product Name: TRIM32

Product Clone Name: 8H8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 105-205

Epitope Target: TRIM32

Epitope Species: human

Product Reactivity/Detection: TRIM32

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for TRIM32 Antibody (8H8): N/A