TRIM3 Antibody (27): sc-136363

TRIM3 Antibody (27) is a mouse monoclonal IgG1 kappa light chain antibody that detects TRIM3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-TRIM3 antibody (27) is available in both non-conjugated and various conjugated forms, including agarose and horseradish peroxidase (HRP). Tripartite motif-containing protein 3 (TRIM3), also known as RING finger protein 22 (RNF22), RING finger protein 97 (RNF97), or brain-expressed RING finger protein (BERP), is a 744 amino acid protein that belongs to the TRIM family, which is characterized by the RING-B-box coiled-coil (RBCC) structure. TRIM3 is primarily localized to cytoplasmic filaments, where TRIM3 plays a crucial role in cell motility and cargo transport by interacting with key components of the actin cytoskeleton, such as α-actinin-4 and myosin V. Specifically, α-actinin-4 binds to the RBCC domain of TRIM3, while the unique C-terminal β-propeller domain of TRIM3 interacts with the C-terminal tail of myosin V. These interactions highlight TRIM3′s importance in cellular dynamics and suggest TRIM3′s potential involvement in various physiological processes. Additionally, TRIM3 has three named isoforms resulting from alternative splicing events, which may contribute to TRIM3′s functional diversity in different cellular contexts.

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TRIM3 Antibody (27) References:

1. Cloning and characterization of a novel RING finger protein that interacts with class V myosins.  |  El-Husseini, AE. and Vincent, SR. 1999. J Biol Chem. 274: 19771-7. PMID: 10391919
2. BERP, a novel ring finger protein, binds to alpha-actinin-4.  |  El-Husseini, AE., et al. 2000. Biochem Biophys Res Commun. 267: 906-11. PMID: 10673389
3. Cloning and characterization of a gene (RNF22) encoding a novel brain expressed ring finger protein (BERP) that maps to human chromosome 11p15.5.  |  El-Husseini, AE., et al. 2001. Genomics. 71: 363-7. PMID: 11170753
4. The tripartite motif family identifies cell compartments.  |  Reymond, A., et al. 2001. EMBO J. 20: 2140-51. PMID: 11331580
5. CART: an Hrs/actinin-4/BERP/myosin V protein complex required for efficient receptor recycling.  |  Yan, Q., et al. 2005. Mol Biol Cell. 16: 2470-82. PMID: 15772161
6. Subclassification of the RBCC/TRIM superfamily reveals a novel motif necessary for microtubule binding.  |  Short, KM. and Cox, TC. 2006. J Biol Chem. 281: 8970-80. PMID: 16434393
7. Solution structure of the RBCC/TRIM B-box1 domain of human MID1: B-box with a RING.  |  Massiah, MA., et al. 2006. J Mol Biol. 358: 532-45. PMID: 16529770
8. Trim-cyclophilin A fusion proteins can restrict human immunodeficiency virus type 1 infection at two distinct phases in the viral life cycle.  |  Yap, MW., et al. 2006. J Virol. 80: 4061-7. PMID: 16571822
9. The RBCC gene RFP2 (Leu5) encodes a novel transmembrane E3 ubiquitin ligase involved in ERAD.  |  Lerner, M., et al. 2007. Mol Biol Cell. 18: 1670-82. PMID: 17314412

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TRIM3 Antibody (27) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-TRIM3 Antibody (27) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TRIM3 Antibody (27) Specifications

Alternate Product Name: TRIM3

Product Clone Name: 27

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 19-128

Epitope Target: TRIM3

Epitope Species: rat

Product Reactivity/Detection: TRIM3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for TRIM3 Antibody (27): AC: Agarose, HRP: Horseradish Peroxidase