Anti-Trichohyalin Antibody (E-11) recommended for detection of Trichohyalin of human origin by WB, IP, IF and ELISA

Anti-Trichohyalin Antibody (E-11): sc-376684

Anti-Trichohyalin Antibody (E-11) is rated 5.0 out of 5 by 2.
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Datasheets

    • Anti-Trichohyalin Antibody (E-11) is a mouse monoclonal IgG1 (kappa light chain) Trichohyalin antibody provided at 200 µg/ml
    • raised against amino acids 23-215 mapping near the N-terminus of Trichohyalin of human origin
    • Trichohyalin Antibody (E-11) is recommended for detection of Trichohyalin of human origin by WB, IP, IF and ELISA
    • Anti-Trichohyalin Antibody (E-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Trichohyalin (E-11): sc-376684.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Trichohyalin Antibody (E-11) for WB applications. These reagents are now offered in bundles with Trichohyalin Antibody (E-11) (see ordering information below).
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Hi. I bought Trichohyalin Antibody (F-2): sc-515130. I need a protocol and kind a sample like positive control for wester blot. Can yuo send me an extract to probe this antibody please. I agree in advance. Thank you.

Asked by: Francisco Sandoval
Thank you for your question. A link to our recommended protocol for Western blotting can be found below. We recommend using HeLa (sc-2200), A-431 (sc-2201) and SW480 (sc-2219) whole cell lysates as positive controls for Western blotting with this antibody. We do not provide sample sizes of our whole cell lysates, but they can be purchased as positive controls. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2019-01-26

What is the recommended starting dilution for Western blot for sc-376684: Trichohyalin (E-11) monoclonal antibody?

Asked by: Germaine
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from Produced positive Western Blot data of Trichohyalin Produced positive Western Blot data of Trichohyalin expression in CCD-1064Sk whole cell lysate and human lung tissue extract . -SCBT QC
Date published: 2015-04-22
Rated 5 out of 5 by from Great Western Blot data of Trichohyalin expression Great Western Blot data of Trichohyalin expression in Hs 732. -SCBT QC
Date published: 2014-04-02
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