TRF1 Antibody (G-7): sc-271485
- TRF1 Antibody (G-7) is a mouse monoclonal IgG1 κ TRF1 antibody, cited in 4 publications, provided at 200 µg/ml
- raised against amino acids 155-396 mapping within an internal region of TRF1 of human origin
- TRF1 Antibody (G-7) is recommended for detection of TRF1 of human origin by WB, IP, IF and ELISA
- Anti-TRF1 Antibody (G-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for TRF1 Antibody (G-7) for WB applications. This reagent is now offered in a bundle with TRF1 Antibody (G-7) (see ordering information below).
QUICK LINKS
TRF1 Antibody (G-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects TRF1 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TRF1 (G-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TRF1, also known as Telomeric Repeat Binding Factor 1, plays a crucial role in maintaining telomere integrity by negatively regulating telomere elongation, which is vital for cellular aging and stability. TRF1 interacts with telomerase, the enzyme responsible for adding repetitive nucleotide sequences to the ends of chromosomes, thereby influencing cellular immortalization and senescence. Proper functioning of TRF1 is essential, as down-regulation in tumor cells can lead to uncontrolled cell proliferation and malignancy. Additionally, TRF1 is characterized by an acidic N-terminus, which is important for interaction with other telomere-associated proteins, such as TRF2, which protects chromosome ends from fusion. Understanding TRF1 dynamics and interactions is critical for elucidating the mechanisms of telomere maintenance and implications for cancer biology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
TRF1 Antibody (G-7) References:
- Role of Pin2/TRF1 in telomere maintenance and cell cycle control. | Zhou, XZ., et al. 2003. J Cell Biochem. 89: 19-37. PMID: 12682905
- Post-translational modifications of TRF1 and TRF2 and their roles in telomere maintenance. | Walker, JR. and Zhu, XD. 2012. Mech Ageing Dev. 133: 421-34. PMID: 22634377
- The splicing factor U2AF65 stabilizes TRF1 protein by inhibiting its ubiquitin-dependent proteolysis. | Kim, J. and Chung, IK. 2014. Biochem Biophys Res Commun. 443: 1124-30. PMID: 24389012
- Dynamics of TRF1 organizing a single human telomere. | Li, X., et al. 2021. Nucleic Acids Res. 49: 760-775. PMID: 33347580
- Structure, dynamics, and regulation of TRF1-TIN2-mediated trans- and cis-interactions on telomeric DNA. | Pan, H., et al. 2021. J Biol Chem. 297: 101080. PMID: 34403696
- TRF1 Depletion Reveals Mutual Regulation Between Telomeres, Kinetochores, and Inner Centromeres in Mouse Oocytes. | Jeon, HJ. and Oh, JS. 2021. Front Cell Dev Biol. 9: 749116. PMID: 34604243
- Assembly path dependence of telomeric DNA compaction by TRF1, TIN2, and SA1. | Liu, M., et al. 2023. Biophys J. 122: 1822-1832. PMID: 37081787
- Structural basis of telomeric nucleosome recognition by shelterin factor TRF1. | Hu, H., et al. 2023. Sci Adv. 9: eadi4148. PMID: 37624885
- Different Role of TRF1 and TRF2 Expression in Non-Small Cell Lung Cancers. | Chae, M., et al. 2024. Onco Targets Ther. 17: 463-469. PMID: 38855632
- TRF1 is a dimer and bends telomeric DNA. | Bianchi, A., et al. 1997. EMBO J. 16: 1785-94. PMID: 9130722
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TRF1 Antibody (G-7) | sc-271485 | 200 µg/ml | $322.00 | |||
TRF1 Antibody (G-7): m-IgG Fc BP-HRP Bundle | sc-525810 | 200 µg Ab; 10 µg BP | $361.00 | |||
TRF1 Antibody (G-7) AC | sc-271485 AC | 500 µg/ml, 25% agarose | $424.00 | |||
TRF1 Antibody (G-7) HRP | sc-271485 HRP | 200 µg/ml | $322.00 | |||
TRF1 Antibody (G-7) FITC | sc-271485 FITC | 200 µg/ml | $336.00 | |||
TRF1 Antibody (G-7) PE | sc-271485 PE | 200 µg/ml | $349.00 | |||
TRF1 Antibody (G-7) Alexa Fluor® 488 | sc-271485 AF488 | 200 µg/ml | $364.00 | |||
TRF1 Antibody (G-7) Alexa Fluor® 546 | sc-271485 AF546 | 200 µg/ml | $364.00 | |||
TRF1 Antibody (G-7) Alexa Fluor® 594 | sc-271485 AF594 | 200 µg/ml | $364.00 | |||
TRF1 Antibody (G-7) Alexa Fluor® 647 | sc-271485 AF647 | 200 µg/ml | $364.00 | |||
TRF1 Antibody (G-7) Alexa Fluor® 680 | sc-271485 AF680 | 200 µg/ml | $364.00 | |||
TRF1 Antibody (G-7) Alexa Fluor® 790 | sc-271485 AF790 | 200 µg/ml | $364.00 |