TRF1 Antibody (TRF-78): sc-56807

TRF1 Antibody (TRF-78) is a mouse monoclonal IgG1 antibody that detects TRF1 of human origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). TRF1 Antibody (TRF-78) is available as the non-conjugated anti-TRF1 antibody (TRF-78) form. The telomeric repeat binding factor 1 (TRF1) plays a crucial role in maintaining telomere integrity by negatively regulating telomere elongation, which is vital for cellular aging and stability. Located at the ends of chromosomes, TRF1 interacts with telomerase, the enzyme responsible for adding repetitive nucleotide sequences to telomeres, thereby influencing cellular immortalization and senescence. By limiting telomere elongation, TRF1 helps prevent excessive cell division, which can lead to tumorigenesis. Additionally, TRF1′s structure includes an acidic N-terminus that is essential for binding affinity and regulatory functions at telomeres. The balance between TRF1 and TRF2, another telomere-binding protein, is critical; while TRF1 restricts telomere length, TRF2 protects chromosome ends from fusion, highlighting the importance of these proteins in genomic stability. Dysregulation of TRF1 expression in cancer cells can contribute to uncontrolled proliferation and malignancy, making TRF1 a significant target for research in cancer biology.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

TRF1 Antibody (TRF-78) References:

1. Immunohistochemical telomeric-repeat binding factor-1 expression in gastrointestinal tumors.  |  Aragona, M., et al. 2000. Oncol Rep. 7: 987-90. PMID: 10948327
2. Expression of telomeric repeat binding factor 1 and 2 and TRF1-interacting nuclear protein 2 in human gastric carcinomas.  |  Matsutani, N., et al. 2001. Int J Oncol. 19: 507-12. PMID: 11494028
3. Telomerase reverse transcriptase and telomeric-repeat binding factor protein 1 as regulators of telomerase activity in pancreatic cancer cells.  |  Yajima, T., et al. 2001. Br J Cancer. 85: 752-7. PMID: 11531263
4. The telomeric poly(ADP-ribose) polymerase, tankyrase 1, contains multiple binding sites for telomeric repeat binding factor 1 (TRF1) and a novel acceptor, 182-kDa tankyrase-binding protein (TAB182).  |  Seimiya, H. and Smith, S. 2002. J Biol Chem. 277: 14116-26. PMID: 11854288
5. Expression of mRNAs for telomeric repeat binding factor (TRF)-1 and TRF2 in atypical adenomatous hyperplasia and adenocarcinoma of the lung.  |  Nakanishi, K., et al. 2003. Clin Cancer Res. 9: 1105-11. PMID: 12631614
6. Expression of the telomeric repeat binding factor gene NgTRF1 is closely coordinated with the cell division program in tobacco BY-2 suspension culture cells.  |  Yang, SW., et al. 2003. J Biol Chem. 278: 21395-407. PMID: 12646586
7. Nucleolar localization of the human telomeric repeat binding factor 2 (TRF2).  |  Zhang, S., et al. 2004. J Cell Sci. 117: 3935-45. PMID: 15265990
8. Expression of telomeric repeat binding factor-1 in astroglial brain tumors.  |  La Torre, D., et al. 2005. Neurosurgery. 56: 802-10. PMID: 15792519
9. Inhibiting TRF1 upstream signaling pathways to target telomeres in cancer cells.  |  Cherfils-Vicini, J. and Gilson, E. 2019. EMBO Mol Med. 11: e10845. PMID: 31273935
10. TRF1 uses a noncanonical function of TFIIH to promote telomere replication.  |  Yang, Z., et al. 2022. Genes Dev. 36: 956-969. PMID: 36229075
11. PARP1 allows proper telomere replication through TRF1 poly (ADP-ribosyl)ation and helicase recruitment.  |  Maresca, C., et al. 2023. Commun Biol. 6: 234. PMID: 36864251
12. TRF1 and TRF2: pioneering targets in telomere-based cancer therapy.  |  Kallingal, A., et al. 2024. J Cancer Res Clin Oncol. 150: 353. PMID: 39012375

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TRF1 Antibody (TRF-78) has 17 citations in a variety of scientific publications.

Primary antibodies like Anti-TRF1 Antibody (TRF-78) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TRF1 Antibody (TRF-78) Specifications

Alternate Product Name: TRF1

Product Clone Name: TRF-78

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids:

Epitope Target: TRF1

Epitope Species: human

Product Reactivity/Detection: TRF1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 100 µg IgG₁ in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 17 citations in various scientific publications

Available Conjugates/Forms for TRF1 Antibody (TRF-78): N/A

The preferred TRF1 antibody is TRF1 Antibody (G-7): sc-271485.