Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
TRAPPC10 CRISPR/Cas9 KO Plasmid (h): sc-406491
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TRAPPC10 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TRAPPC10 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TRAPPC10 Antibody (RR-18): sc-101259
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TRAPPC10 CRISPR/Cas9 KO Plasmid (h) | sc-406491 | 20 µg | $397.00 |
Overview
TRAPPC10 encodes a core subunit of the TRAPP (transport protein particle) complex that supports vesicle tethering and membrane trafficking across the early secretory and endomembrane system. Through its role in coordinating vesicular transport and Golgi/ER-associated trafficking steps, TRAPPC10 contributes to intracellular protein sorting, organelle homeostasis, and cargo delivery required for normal cell physiology. Disruption of TRAPP complex components can perturb secretory pathway flux and stress responses, making TRAPPC10 relevant for mechanistic studies of trafficking-dependent signaling and cellular maintenance programs. Altered vesicle transport is also implicated in neurodevelopmental and neurodegenerative phenotypes, positioning TRAPPC10 as a useful target for modeling trafficking-linked disease mechanisms in human cells.
TRAPPC10 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TRAPPC10 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TRAPPC10 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TRAPPC10 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TRAPPC10 protein expression.
This CRISPR knockout system enables efficient generation of TRAPPC10-deficient cell models for investigation of TRAPPC10 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TRAPPC10 exon(s) critical for TRAPPC10 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TRAPPC10 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TRAPPC10 CRISPR/Cas9 KO Plasmid (h) and TRAPPC10 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TRAPPC10 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TRAPPC10 HDR Plasmid (h) and TRAPPC10 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TRAPPC10 homology arms to support homology-directed repair at defined TRAPPC10 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.