TRAP230 Antibody (E-2): sc-515695

TRAP230 Antibody (E-2) is a mouse monoclonal IgG1 antibody that detects TRAP230 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TRAP230 (E-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TRAP230 protein plays a crucial role in regulating transcription within mammalian cells, functioning as part of the thyroid hormone receptor-associated protein (TRAP) complex, which is essential for mediating interaction between transcriptional activators and initiation factors. This complex is vital for hormone-induced transcriptional activation, as TRAP230 interacts with nuclear receptors such as thyroid hormone receptors and vitamin D receptors. The TRAP complex is composed of several proteins, including TRAP95, TRAP100, TRAP150, TRAP220, and TRAP230, all containing a nuclear receptor recognition motif that facilitates ligand-dependent binding to these receptors. TRAP220 and TRAP100 are predominantly expressed in skeletal muscle, heart, and placenta, highlighting their importance in tissue-specific gene regulation. TRAP95, TRAP150, and TRAP230 enhance transcriptional activity by associating with the SMCC (SRB and MED protein cofactor complex), which shares homology with components of yeast transcriptional mediator complexes, underscoring the evolutionary conservation of transcriptional regulation mechanisms.

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TRAP230 Antibody (E-2) References:

1. A novel human SRB/MED-containing cofactor complex, SMCC, involved in transcription regulation.  |  Gu, W., et al. 1999. Mol Cell. 3: 97-108. PMID: 10024883
2. Competition between thyroid hormone receptor-associated protein (TRAP) 220 and transcriptional intermediary factor (TIF) 2 for binding to nuclear receptors. Implications for the recruitment of TRAP and p160 coactivator complexes.  |  Treuter, E., et al. 1999. J Biol Chem. 274: 6667-77. PMID: 10037764
3. Identity between TRAP and SMCC complexes indicates novel pathways for the function of nuclear receptors and diverse mammalian activators.  |  Ito, M., et al. 1999. Mol Cell. 3: 361-70. PMID: 10198638
4. Identification of mouse TRAP100: a transcriptional coregulatory factor for thyroid hormone and vitamin D receptors.  |  Zhang, J. and Fondell, JD. 1999. Mol Endocrinol. 13: 1130-40. PMID: 10406464
5. The structure of the nuclear hormone receptors.  |  Kumar, R. and Thompson, EB. 1999. Steroids. 64: 310-9. PMID: 10406480
6. The TRAP220 component of a thyroid hormone receptor- associated protein (TRAP) coactivator complex interacts directly with nuclear receptors in a ligand-dependent fashion.  |  Yuan, CX., et al. 1998. Proc Natl Acad Sci U S A. 95: 7939-44. PMID: 9653119
7. Mammalian mediator of transcriptional regulation and its possible role as an end-point of signal transduction pathways.  |  Jiang, YW., et al. 1998. Proc Natl Acad Sci U S A. 95: 8538-43. PMID: 9671713

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TRAP230 Antibody (E-2) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-TRAP230 Antibody (E-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TRAP230 Antibody (E-2) Specifications

Alternate Product Name: TRAP230

Product Clone Name: E-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids: 1974-1997

Epitope Target: TRAP230

Epitope Species: human

Product Reactivity/Detection: TRAP230

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for TRAP230 Antibody (E-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent