TRAP150 Antibody (A-11): sc-133249

TRAP150 Antibody (A-11) is a mouse monoclonal IgG2b antibody that detects TRAP150 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). TRAP150 plays a crucial role in the regulation of transcription by acting as a coactivator within the thyroid hormone receptor-associated protein (TRAP) complex, which is essential for mediating the interaction between transcriptional activators and transcriptional machinery. TRAP150 is primarily located in the nucleus, where TRAP150 facilitates hormone-induced transcriptional activation by binding to nuclear receptors, such as thyroid hormone receptors and vitamin D receptors. TRAP150′s presence in the nucleus is vital for proper regulation of gene expression in response to hormonal signals, which can influence various physiological processes including metabolism, development, and cellular differentiation. TRAP150 interacts with other components of the TRAP complex, such as TRAP95 and TRAP220, enhancing the overall transcriptional coactivation process. TRAP150′s ability to form complexes with these proteins underscores TRAP150′s importance in the intricate network of transcriptional regulation, making TRAP150 a valuable target for research in gene expression and hormonal signaling pathways.

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TRAP150 Antibody (A-11) References:

1. TRAP150 activates pre-mRNA splicing and promotes nuclear mRNA degradation.  |  Lee, KM., et al. 2010. Nucleic Acids Res. 38: 3340-50. PMID: 20123736
2. Phosphorylation-dependent regulation of PSF by GSK3 controls CD45 alternative splicing.  |  Heyd, F. and Lynch, KW. 2010. Mol Cell. 40: 126-37. PMID: 20932480
3. Btf and TRAP150 have distinct roles in regulating subcellular mRNA distribution.  |  Varia, S., et al. 2013. Nucleus. 4: 229-40. PMID: 23778535
4. A positive feedback loop links circadian clock factor CLOCK-BMAL1 to the basic transcriptional machinery.  |  Lande-Diner, L., et al. 2013. Proc Natl Acad Sci U S A. 110: 16021-6. PMID: 24043798
5. TRAP150 activates splicing in composite terminal exons.  |  Lee, KM. and Tarn, WY. 2014. Nucleic Acids Res. 42: 12822-32. PMID: 25326322
6. TRAP150 interacts with the RNA-binding domain of PSF and antagonizes splicing of numerous PSF-target genes in T cells.  |  Yarosh, CA., et al. 2015. Nucleic Acids Res. 43: 9006-16. PMID: 26261210
7. Alignment of Mitotic Chromosomes in Human Cells Involves SR-Like Splicing Factors Btf and TRAP150.  |  Varia, S., et al. 2017. Int J Mol Sci. 18: PMID: 28895891
8. The DNA damage response activates HPV16 late gene expression at the level of RNA processing.  |  Nilsson, K., et al. 2018. Nucleic Acids Res. 46: 5029-5049. PMID: 29596642
9. High-throughput identification of noncoding functional SNPs via type IIS enzyme restriction.  |  Li, G., et al. 2018. Nat Genet. 50: 1180-1188. PMID: 30013183
10. A novel HPV16 splicing enhancer critical for viral oncogene expression and cell immortalization.  |  Jönsson, J., et al. 2024. Nucleic Acids Res. 52: 316-336. PMID: 37994701

The preferred TRAP150 antibody is TRAP150 Antibody (F-10): sc-133250.