Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Transketolase CRISPR/Cas9 KO Plasmid (m): sc-423410
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Transketolase CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Transketolase genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Transketolase Antibody (H-7): sc-390179
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Transketolase CRISPR/Cas9 KO Plasmid (m) | sc-423410 | 20 µg | $397.00 |
Overview
Mouse Tkt encodes transketolase, a thiamine pyrophosphate–dependent enzyme that catalyzes reversible carbon transfer reactions in the non-oxidative branch of the pentose phosphate pathway. By interconverting sugar phosphates, transketolase supports ribose-5-phosphate production for nucleotide biosynthesis and helps balance glycolytic and pentose phosphate intermediates to sustain cellular redox and anabolic demands. TKT activity is therefore linked to proliferative metabolism, mitochondrial and oxidative stress adaptation via NADPH-dependent processes, and broader carbohydrate flux control. Altered pentose phosphate pathway routing and transketolase expression have been associated with metabolic dysregulation and tumor-associated metabolic phenotypes, making Tkt a useful target for mechanistic studies of metabolic remodeling.
Transketolase CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Tkt gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Tkt together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Tkt open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Transketolase protein expression.
This CRISPR knockout system enables efficient generation of Tkt-deficient cell models for investigation of Transketolase signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Tkt exon(s) critical for Transketolase function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Tkt genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Transketolase CRISPR/Cas9 KO Plasmid (m) and Transketolase CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Tkt locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Transketolase HDR Plasmid (m) and Transketolase HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Tkt homology arms to support homology-directed repair at defined Tkt target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.