TRAF6 Antibody (D-10): sc-8409

TRAF6 Antibody (D-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects the TRAF6 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). TRAF6 (D-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Tumor necrosis factor receptor-associated factor 6 (TRAF6) plays a critical role in regulating adaptive immunity, innate immunity, and bone metabolism, making TRAF6 essential for maintaining immune homeostasis and skeletal integrity. As a ubiquitin ligase, TRAF6 is pivotal in mediating the activation of key protein kinases, such as transforming growth factor β-activated kinase (TAK1) and IκB kinase (IKK), through the formation of a unique polyubiquitin chain linked via Lys 63 of ubiquitin. This post-translational modification is crucial for the activation of the NFκB signaling pathway in response to interleukin-1 and Toll-like receptor ligands, highlighting TRAF6′s importance in immune signaling. The coiled-coil domain of TRAF6 is particularly significant for TRAF6 auto-ubiquitination and subsequent activation of the NFκB pathway. Furthermore, TRAF6 interacts with various protein kinases, including IRAK1/IRAK, SRC, and PKCΩ, establishing a connection between distinct signaling pathways that are vital for cellular responses to stress and inflammation.

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TRAF6 Antibody (D-10) References:

1. Interaction of TRAF6 with MAST205 regulates NF-kappaB activation and MAST205 stability.  |  Xiong, H., et al. 2004. J Biol Chem. 279: 43675-83. PMID: 15308666
2. The coiled-coil domain of TRAF6 is essential for its auto-ubiquitination.  |  Yang, K., et al. 2004. Biochem Biophys Res Commun. 324: 432-9. PMID: 15465037
3. TIFA activates IkappaB kinase (IKK) by promoting oligomerization and ubiquitination of TRAF6.  |  Ea, CK., et al. 2004. Proc Natl Acad Sci U S A. 101: 15318-23. PMID: 15492226
4. The role of TRAF6 in signal transduction and the immune response.  |  Kobayashi, T., et al. 2004. Microbes Infect. 6: 1333-8. PMID: 15555541
5. Interleukin-8 induces nuclear transcription factor-kappaB through a TRAF6-dependent pathway.  |  Manna, SK. and Ramesh, GT. 2005. J Biol Chem. 280: 7010-21. PMID: 15591054
6. RANK-mediated amplification of TRAF6 signaling leads to NFATc1 induction during osteoclastogenesis.  |  Gohda, J., et al. 2005. EMBO J. 24: 790-9. PMID: 15678102
7. Strength of TRAF6 signalling determines osteoclastogenesis.  |  Kadono, Y., et al. 2005. EMBO Rep. 6: 171-6. PMID: 15678156
8. The pro-atherogenic cytokine interleukin-18 induces CXCL16 expression in rat aortic smooth muscle cells via MyD88, interleukin-1 receptor-associated kinase, tumor necrosis factor receptor-associated factor 6, c-Src, phosphatidylinositol 3-kinase, Akt, c-Jun N-terminal kinase, and activator protein-1 signaling.  |  Chandrasekar, B., et al. 2005. J Biol Chem. 280: 26263-77. PMID: 15890643
9. Activation of IKK by thymosin alpha1 requires the TRAF6 signalling pathway.  |  Zhang, P., et al. 2005. EMBO Rep. 6: 531-7. PMID: 15905851
10. The E3 ligase TRAF6 regulates Akt ubiquitination and activation.  |  Yang, WL., et al. 2009. Science. 325: 1134-8. PMID: 19713527
11. Inhibition of TRAF6 ubiquitin-ligase activity by PRDX1 leads to inhibition of NFKB activation and autophagy activation.  |  Min, Y., et al. 2018. Autophagy. 14: 1347-1358. PMID: 29929436
12. K48/K63-linked polyubiquitination of ATG9A by TRAF6 E3 ligase regulates oxidative stress-induced autophagy.  |  Wang, YT., et al. 2022. Cell Rep. 38: 110354. PMID: 35196483

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TRAF6 Antibody (D-10) has 244 citations in a variety of scientific publications.

Primary antibodies like Anti-TRAF6 Antibody (D-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TRAF6 Antibody (D-10) Specifications

Alternate Product Name: TRAF6

Product Clone Name: D-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-274

Epitope Target: TRAF6

Epitope Species: human

Product Reactivity/Detection: TRAF6

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 244 citations in various scientific publications

Available Conjugates/Forms for TRAF6 Antibody (D-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin