Date published: 2025-9-5
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TRAF3 Antibody (G-6) - Western Blotting - Image 396701
TRAF3 抗体 (G-6) HRP: sc-6933 HRP. NIH/3T3 (A), IB4 (B), TRAF 转染的 L53. 3 IB4 (C) 和 TRAF 转染的 L75. 11 IB4 (D) 全细胞裂解液中 TRAF3 表达的直接 Western 印迹分析.
TRAF3 抗体 (G-6) HRP: sc-6933 HRP. NIH/3T3 (A), IB4 (B), TRAF 转染的 L53. 3 IB4 (C) 和 TRAF 转染的 L75. 11 IB4 (D) 全细胞裂解液中 TRAF3 表达的直接 Western 印迹分析.

TRAF3 抗体 (G-6): sc-6933

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寫評論提問

说明书
  • TRAF3 抗体 G-6 是小鼠单克隆 IgG2b κ，TRAF3抗体, 在41篇文献中引用，规格为200 µg/ml
  • 免疫human来源TRAF3近C-terminus的322-444氨基酸
  • TRAF3 抗体 (G-6) 推荐用于 WB, IP, IF 和 FCM，检测mouse, rat 和human 来源的 TRAF3
  • 抗TRAF3抗体（G-6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取TRAF3 (G-6): sc-6933的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 2b BP-HRP">m-IgG2b BP-HRP是TRAF3 Antibody (G-6) 适用于 WB 应用。 的首选辅助检测试剂，这些试剂现与TRAF3 Antibody (G-6) 打包提供（请参阅下面的订购信息）。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

额外TRAF抗体，包括TRAF1, TRAF2, TRAF3, TRAF4, TRAF5和TRAF6

TRAF3抗体（G-6）是一种小鼠单克隆IgG2b kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和流式细胞术（FCM）检测小鼠、大鼠和人类来源的TRAF3蛋白。TRAF3单克隆抗体（G-6）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。TRAF3蛋白在调节免疫反应和细胞信号传导通路中发挥着关键作用，尤其是在肿瘤坏死因子（TNF）受体介导的信号传导中。TRAF3主要参与信号复合物的形成，调节核因子κ-轻链激活的活化B细胞（NF-κB）和促分裂原活化蛋白激酶（MAPK）通路的激活，这些通路对于免疫反应和炎症至关重要。TRAF3的正常运作至关重要，因为失调会导致各种疾病，包括自身免疫性疾病和癌症。TRAF3与TNF受体超家族中的其他蛋白（如TRAF1和TRAF2）相互作用，形成异二聚体复合物，从而提高信号传导效率。TRAF3在这些通路中的作用得到确认，这凸显了其在维持细胞稳态和对外部刺激作出反应方面的重要性，使得TRAF3单克隆抗体（G-6）成为研究免疫信号传导和相关病理的研究人员的重要工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

TRAF3 抗体 (G-6) 参考文献:

  1. 两种 TNF 受体  |  Tartaglia, LA. and Goeddel, DV. 1992. Immunol Today. 13: 151-3. PMID: 1322675
  2. TRAF3、泛素化和B淋巴细胞调节。  |  Lin, WW., et al. 2015. Immunol Rev. 266: 46-55. PMID: 26085206
  3. TRAF3作为B淋巴细胞存活和活化的多面调节器。  |  Bishop, GA., et al. 2018. Front Immunol. 9: 2161. PMID: 30319624
  4. TRAF3激活STING介导的EV-A71抑制和病毒逃逸靶点。  |  Zheng, W., et al. 2023. Signal Transduct Target Ther. 8: 79. PMID: 36823147
  5. TRAF3-EWSR1信号轴可作为生发中心反应的检查点。  |  Li, Y., et al. 2023. J Exp Med. 220: PMID: 37097293
  6. TRAF3功能丧失揭示了非典型NF-κB通路作为弥漫性大B细胞淋巴瘤的治疗靶点。  |  Li, MY., et al. 2024. Proc Natl Acad Sci U S A. 121: e2320421121. PMID: 38662551
  7. 一种新型 RING 手指蛋白与 CD40 的细胞质结构域相互作用。  |  Hu, HM., et al. 1994. J Biol Chem. 269: 30069-72. PMID: 7527023
  8. CRAF1是TRAF的亲戚，它参与了CD40信号传导。  |  Cheng, G., et al. 1995. Science. 267: 1494-8. PMID: 7533327
  9. TNF 受体 1 相关蛋白 TRADD 发出细胞死亡和 NF-kappa B 激活信号。  |  Hsu, H., et al. 1995. Cell. 81: 495-504. PMID: 7758105
  10. Epstein-Barr 病毒转化蛋白 LMP1 与肿瘤坏死因子受体家族的信号蛋白结合。  |  Mosialos, G., et al. 1995. Cell. 80: 389-99. PMID: 7859281
  11. 与 75 kDa 肿瘤坏死因子受体胞质结构域缔合作用的新型假定信号转导子家族。  |  Rothe, M., et al. 1994. Cell. 78: 681-92. PMID: 8069916
  12. 细胞和病毒蛋白 TNF 受体超家族：激活, 成本刺激和死亡。  |  Smith, CA., et al. 1994. Cell. 76: 959-62. PMID: 8137429

订购信息

产品名称产品编号规格价格数量收藏夹

TRAF3 抗体 (G-6)

sc-6933
200 µg/ml
$316.00

TRAF3 (G-6): m-IgG Fc BP-HRP 套装

sc-525431
200 µg Ab; 10 µg BP
$354.00

TRAF3 (G-6): m-IgG2b BP-HRP 套装

sc-549561
200 µg Ab; 10 µg BP
$354.00

TRAF3 抗体 (G-6) AC

sc-6933 AC
500 µg/ml, 25% agarose
$416.00

TRAF3 抗体 (G-6) HRP

sc-6933 HRP
200 µg/ml
$316.00

TRAF3 抗体 (G-6) FITC

sc-6933 FITC
200 µg/ml
$330.00

TRAF3 抗体 (G-6) PE

sc-6933 PE
200 µg/ml
$343.00

TRAF3 抗体 (G-6) Alexa Fluor® 488

sc-6933 AF488
200 µg/ml
$357.00

TRAF3 抗体 (G-6) Alexa Fluor® 546

sc-6933 AF546
200 µg/ml
$357.00

TRAF3 抗体 (G-6) Alexa Fluor® 594

sc-6933 AF594
200 µg/ml
$357.00

TRAF3 抗体 (G-6) Alexa Fluor® 647

sc-6933 AF647
200 µg/ml
$357.00

TRAF3 抗体 (G-6) Alexa Fluor® 680

sc-6933 AF680
200 µg/ml
$357.00

TRAF3 抗体 (G-6) Alexa Fluor® 790

sc-6933 AF790
200 µg/ml
$357.00

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with TRAF3 Antibody (G-6): sc-6933?

Asked by: jenni
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 3
Date published: 2017-01-09
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Rated 2 out of 5 by from Non rilevata immunogenicità nel caneAbbiamo testato il campione di prova in immunoistochimica su tessuti di cane ma non abbiamo ottenuto un segnale specifico.
Date published: 2022-05-20
Rated 5 out of 5 by from Good bands in western blot.I use 1:200 in mice primary macrophage cell lysates. The bands look good.
Date published: 2020-09-29
Rated 1 out of 5 by from not specific for TRAF3This is a terrible antibody that doesn't recognize TRAF3. Despite having been raised against a similar region (per the website) of TRAF3 as a TRAF3 Ab (sc-1828) that has since been discontinued, sc-6933 appears to detect a protein that is not TRAF3 but is (distressingly) the same size as TRAF3, because it shows up in the TRAF3-/- cells. The image is a blot from lysates of a human T cell line without and with TRAF3, using the old TRAF3 Ab and the current "improved" TRAF3 Ab. This TRAF3-/- cell line has been extensively validated as a true and complete TRAF3 knockout. We see similar results in primary mouse cells and cell lines. The bottom line: trash antibody please bring back the old one!
Date published: 2018-07-21
Rated 5 out of 5 by from Remarkable resultsTRAF3 Antibody (G-6): sc-6933 detection, WB has a very strong and clear signals in 1:1000 and is very obvious
Date published: 2017-10-20
Rated 5 out of 5 by from Good WB resultTRAF3 play an important role in innate immunity, we detected expression in mouse brain using this antibody, WB resust showed this antibody is good performance, 1:1000 diluted got very clear, no non-specific band and background.
Date published: 2017-08-12
Rated 5 out of 5 by from Good antibody.We used this antibody in IF in human cells and got positive results. It's very good antibody. Recommend dilution is 1:100~1:200.
Date published: 2017-06-22
Rated 5 out of 5 by from was usefulWe used this antibody in IF in mouse brain tissues and got positive staining, very good antibody.
Date published: 2017-05-07
Rated 5 out of 5 by from Western BlottingWB for TRAF3 analysis, recommend use 1:200 diluiton, the band is good and specific.
Date published: 2017-04-22
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TRAF3 抗体 (G-6) is rated 4.6 out of 5 by 18.
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