Anti-Tra-2 beta Antibody (D-2) recommended for detection of Tra-2β of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-Tra-2β Antibody (D-2): sc-166829

Anti-Tra-2β Antibody (D-2) is rated 4.0 out of 5 by 4.
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Datasheets

    • Anti-Tra-2β Antibody (D-2) is a mouse monoclonal IgG2a κ Tra-2β antibody, cited in 3 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 19-36 near the N-terminus of Tra-2β of human origin
    • Anti-Tra-2 beta Antibody (D-2) is recommended for detection of Tra-2β of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-Tra-2 beta Antibody (D-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for ChIP application (sc-166829 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Tra-2β (D-2): sc-166829.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for Tra-2β Antibody (D-2) for WB and IHC(P) applications. These reagents are now offered in bundles with Tra-2β Antibody (D-2) (see ordering information below).
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What application is the blocking peptide sc-166829 P appropriate for?

Asked by: DefinitelyNotMatt
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-27
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Rated 1 out of 5 by from Weak antibody Very weak antibody. Band visible at 1:100 dilution only after 1-hour exposition on film. Tested in a HCT116 whole cell lysate.
Date published: 2018-09-03
Rated 5 out of 5 by from Good nuclear staining on several cancer cell lines Nice and clean staining on several cancer cell lines and clean sharp bands on western blot
Date published: 2017-11-28
Rated 5 out of 5 by from Great immunofluorescence nuclear staining Great immunofluorescence nuclear staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-02-17
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