Tra-2β Antibody (D-2): sc-166829
- Tra-2β Antibody (D-2) is a mouse monoclonal IgG2a κ Tra-2β antibody, cited in 4 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 19-36 near the N-terminus of Tra-2β of human origin
- Tra-2 beta Antibody (D-2) is recommended for detection of Tra-2β of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-Tra-2 beta Antibody (D-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-166829 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Tra-2β Antibody (D-2) for WB and IHC(P) applications. These reagents are now offered in bundles with Tra-2β Antibody (D-2) (see ordering information below).
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Tra-2β Antibody (D-2) is a mouse monoclonal IgG2a kappa light chain antibody that detects Tra-2 beta protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-Tra-2β antibody (D-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Tra-2 beta protein plays a crucial role in regulating pre-mRNA splicing, particularly in forming Tra-2/SR complexes with serine/arginine-rich splicing factors, which are essential for tissue-specific alternative splicing of numerous pre-mRNAs. This interaction is vital for proper expression of genes involved in various biological processes, including development and differentiation. Notably, Tra-2β expression increases during reoxygenation of hypoxic astrocytes, highlighting a potential role in cellular responses to stress and injury. Tra-2 proteins selectively bind to purine-rich sequences through RNP-type RNA-binding domains, underscoring their importance in splicing machinery and making Tra-2β a significant target for studies related to gene regulation and splicing mechanisms in both normal physiology and disease states.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Tra-2β Antibody (D-2) References:
- Cloning of a novel RNA binding polypeptide (RA301) induced by hypoxia/reoxygenation. | Matsuo, N., et al. 1995. J Biol Chem. 270: 28216-22. PMID: 7499316
- The role of specific protein-RNA and protein-protein interactions in positive and negative control of pre-mRNA splicing by Transformer 2. | Amrein, H., et al. 1994. Cell. 76: 735-46. PMID: 8124712
- Molecular cloning of a mouse homologue for the Drosophila splicing regulator Tra2. | Segade, F., et al. 1996. FEBS Lett. 387: 152-6. PMID: 8674539
- A human homologue of the Drosophila sex determination factor transformer-2 has conserved splicing regulatory functions. | Dauwalder, B., et al. 1996. Proc Natl Acad Sci U S A. 93: 9004-9. PMID: 8799144
- Molecular cloning of htra2-beta-1 and htra2-beta-2, two human homologs of tra-2 generated by alternative splicing. | Beil, B., et al. 1997. DNA Cell Biol. 16: 679-90. PMID: 9212162
- Human Tra2 proteins are sequence-specific activators of pre-mRNA splicing. | Tacke, R., et al. 1998. Cell. 93: 139-48. PMID: 9546399
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Tra-2β Antibody (D-2) | sc-166829 | 200 µg/ml | $322.00 | |||
Tra-2β Antibody (D-2): m-IgG Fc BP-HRP Bundle | sc-529062 | 200 µg Ab; 10 µg BP | $361.00 | |||
Tra-2β Antibody (D-2): m-IgGκ BP-HRP Bundle | sc-521761 | 200 µg Ab, 40 µg BP | $361.00 | |||
Tra-2β Antibody (D-2): m-IgG2a BP-HRP Bundle | sc-547252 | 200 µg Ab; 10 µg BP | $361.00 | |||
Tra-2β Antibody (D-2) X | sc-166829 X | 200 µg/0.1 ml | $322.00 | |||
Tra-2β Antibody (D-2) AC | sc-166829 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Tra-2β Antibody (D-2) HRP | sc-166829 HRP | 200 µg/ml | $322.00 | |||
Tra-2β Antibody (D-2) FITC | sc-166829 FITC | 200 µg/ml | $336.00 | |||
Tra-2β Antibody (D-2) PE | sc-166829 PE | 200 µg/ml | $349.00 | |||
Tra-2β Antibody (D-2) Alexa Fluor® 488 | sc-166829 AF488 | 200 µg/ml | $364.00 | |||
Tra-2β Antibody (D-2) Alexa Fluor® 546 | sc-166829 AF546 | 200 µg/ml | $364.00 | |||
Tra-2β Antibody (D-2) Alexa Fluor® 594 | sc-166829 AF594 | 200 µg/ml | $364.00 | |||
Tra-2β Antibody (D-2) Alexa Fluor® 647 | sc-166829 AF647 | 200 µg/ml | $364.00 | |||
Tra-2β Antibody (D-2) Alexa Fluor® 680 | sc-166829 AF680 | 200 µg/ml | $364.00 | |||
Tra-2β Antibody (D-2) Alexa Fluor® 790 | sc-166829 AF790 | 200 µg/ml | $364.00 | |||
Tra-2β (D-2) Neutralizing Peptide | sc-166829 P | 100 µg/0.5 ml | $69.00 |