TRA-1-81 Antibody (TRA-1-80): sc-21706

TRA-1-81 Antibody (TRA-1-80) is a mouse monoclonal IgM kappa light chain antibody that specifically recognizes the high molecular weight glycoprotein podocalyxin-like protein 1 (PODXL), also known as PCLP1, gp200, and thrombomucin. The TRA-1-81 antigen is a carbohydrate epitope expressed on podocalyxin, a type I transmembrane glycoprotein. PODXL monoclonal antibody (TRA-1-80), raised against 2102Ep human embryonal carcinoma cells, is reactive with human TRA-1-81 antigen by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). PODXL is predominantly located on the apical membrane of cells, particularly in the kidney′s glomerular podocytes, playing a crucial role in maintaining the filtration barrier, as well as in cell adhesion and migration. PODXL expression on the cell surface is a key marker for undifferentiated human embryonic stem cells and embryonal carcinoma cells. TRA-1-81 expression is lost upon stem cell differentiation, making PODXL monoclonal antibody (TRA-1-80) an important marker for identifying undifferentiated cells. Anti-PODXL antibody (TRA-1-80) is instrumental in identifying pluripotent stem cells in vitro, often used alongside other stem cell markers such as TRA-1-60 and Stage-Specific Embryonic Antigens (SSEA-3 and SSEA-4). PODXL (TRA-1-80) antibody reacts with a high-molecular-mass carbohydrate epitope on the surface of human embryonal carcinoma (EC), embryonic germ (EG), embryonic stem (ES), and induced pluripotent stem (iPS) cells. Anti-PODXL antibody (TRA-1-80) is available in both non-conjugated form and various conjugated forms, including phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different applications. TRA-1-81 antigen detection is essential for stem cell research and for identifying embryonal carcinoma positive nonseminomatous testicular germ cell tumors (NSTGCT), where PODXL is co-expressed with TRA-1-60 and SSEAs on the membrane of numerous stem cells. PODXL monoclonal antibody (TRA-1-80) serves as a valuable tool in stem cell biology and cancer research, facilitating the study of cell surface markers involved in cell differentiation, adhesion, and tumorigenesis.

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TRA-1-81 Antibody (TRA-1-80) References:

1. The human embryonal carcinoma marker antigen TRA-1-60 is a sialylated keratan sulfate proteoglycan.  |  Badcock, G., et al. 1999. Cancer Res. 59: 4715-9. PMID: 10493530
2. The TRA-1-60 and TRA-1-81 human pluripotent stem cell markers are expressed on podocalyxin in embryonal carcinoma.  |  Schopperle, WM. and DeWolf, WC. 2007. Stem Cells. 25: 723-30. PMID: 17124010
3. TRA-1-60: a new serum marker in patients with germ-cell tumors.  |  Marrink, J., et al. 1991. Int J Cancer. 49: 368-72. PMID: 1717384
4. Immunohistochemical localisation of TRA-1-60, TRA-1-81, GCTM-2 and podocalyxin in the developing baboon kidney.  |  Gubhaju, L., et al. 2008. Histochem Cell Biol. 129: 651-7. PMID: 18265998
5. The binding specificity of the marker antibodies Tra-1-60 and Tra-1-81 reveals a novel pluripotency-associated type 1 lactosamine epitope.  |  Natunen, S., et al. 2011. Glycobiology. 21: 1125-30. PMID: 21159783
6. Human embryonal carcinoma cells and their differentiation in culture.  |  Andrews, PW., et al. 1987. Int J Androl. 10: 95-104. PMID: 2438234
7. A targetable pathway to eliminate TRA-1-60+/TRA-1-81+ chemoresistant cancer cells.  |  Tan, L., et al. 2023. J Mol Cell Biol. 15: PMID: 37327088
8. Isolation of a primate embryonic stem cell line.  |  Thomson, JA., et al. 1995. Proc Natl Acad Sci U S A. 92: 7844-8. PMID: 7544005
9. Retinoic acid-induced differentiation of the developmentally pluripotent human germ cell tumor-derived cell line, NCCIT.  |  Damjanov, I., et al. 1993. Lab Invest. 68: 220-32. PMID: 7680083
10. Comparative analysis of cell surface antigens expressed by cell lines derived from human germ cell tumours.  |  Andrews, PW., et al. 1996. Int J Cancer. 66: 806-16. PMID: 8647654
11. Pluripotent cell lines derived from common marmoset (Callithrix jacchus) blastocysts.  |  Thomson, JA., et al. 1996. Biol Reprod. 55: 254-9. PMID: 8828827
12. Importance of a new tumor marker TRA-1-60 in the follow-up of patients with clinical stage I nonseminomatous testicular germ cell tumors.  |  Gels, ME., et al. 1997. Ann Surg Oncol. 4: 321-7. PMID: 9181232

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TRA-1-81 Antibody (TRA-1-80) has 130 citations in a variety of scientific publications.

Primary antibodies like Anti-TRA-1-81 Antibody (TRA-1-80) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TRA-1-81 Antibody (TRA-1-80) Specifications

Alternate Product Name: Podocalyxin

Product Clone Name: TRA-1-80

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids:

Epitope Target: TRA-1-81

Epitope Species: human

Product Reactivity/Detection: TRA-1-81

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, FCM

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 130 citations in various scientific publications

Available Conjugates/Forms for TRA-1-81 Antibody (TRA-1-80): AF647: Alexa Fluor^® 647