Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
TR2 CRISPR/Cas9 KO Plasmid (m): sc-423489
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TR2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TR2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TR2 Antibody (D-4): sc-374049
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TR2 CRISPR/Cas9 KO Plasmid (m) | sc-423489 | 20 µg | $397.00 |
Overview
Mouse Nr2c1 encodes the orphan nuclear receptor TR2, a sequence-specific transcription factor that binds hormone response elements and modulates gene expression programs linked to cell fate decisions. TR2 participates in nuclear receptor signaling and transcriptional coregulator networks that influence differentiation, proliferation, and metabolic homeostasis, with prominent roles reported in germ cell development and pluripotency-associated regulatory circuits. Through these transcriptional functions, NR2C1 can affect chromatin state and lineage commitment pathways that are frequently perturbed in developmental abnormalities and cancer-related transcriptional dysregulation. Accordingly, Nr2c1 is a useful target for dissecting gene regulatory mechanisms underlying stem-like states and tissue-specific differentiation in mouse models.
TR2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Nr2c1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Nr2c1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Nr2c1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TR2 protein expression.
This CRISPR knockout system enables efficient generation of Nr2c1-deficient cell models for investigation of TR2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Nr2c1 exon(s) critical for TR2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Nr2c1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TR2 CRISPR/Cas9 KO Plasmid (m) and TR2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Nr2c1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TR2 HDR Plasmid (m) and TR2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Nr2c1 homology arms to support homology-directed repair at defined Nr2c1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.