TPN Antibody (TO-3): sc-80647
- TPN Antibody (TO-3) is a mouse monoclonal IgG1 κ, cited in 6 publications, provided at 200 µg/ml
- raised against a synthetic peptide corresponding to amino acids 49-62 of TPN of human origin
- Anti-TPN Antibody (TO-3) is recommended for detection of TPN of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM
- Anti-TPN Antibody (TO-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for TPN Antibody (TO-3) for WB and IHC(P) applications. These reagents are now offered in bundles with TPN Antibody (TO-3) (see ordering information below).
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SEE ALSO...
TPN Antibody (TO-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects TPN in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). TPN Antibody (TO-3) is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TPN, also known as tapasin, TPSN, TAPBP, or transporter associated with antigen processing-A, is a type I membrane glycoprotein encoded by a gene located on chromosome 6p21, producing a 488-residue protein. TPN plays a crucial role in the immune response by facilitating the transfer of peptide antigens onto major histocompatibility complex (MHC) class I molecules, which is essential for the presentation of antigens to CD8+ T cells. The cytoplasmic domain of TPN contains a double-lysine motif that is critical for retention in the endoplasmic reticulum, ensuring newly assembled MHC class I molecules are properly loaded with antigens before transport to the cell surface. TPN knockout mice exhibit significant defects in MHC class I expression and antigen presentation, underscoring TPN′s importance in maintaining effective immune responses.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
TPN Antibody (TO-3) References:
- Peptide-bound major histocompatibility complex class I molecules associate with tapasin before dissociation from transporter associated with antigen processing. | Li, S., et al. 1999. J Biol Chem. 274: 8649-54. PMID: 10085102
- Tapasin is required for efficient peptide binding to transporter associated with antigen processing. | Li, S., et al. 2000. J Biol Chem. 275: 1581-6. PMID: 10636848
- Regulation of transporter associated with antigen processing by phosphorylation. | Li, Y., et al. 2000. J Biol Chem. 275: 24130-5. PMID: 10823836
- Impaired assembly yet normal trafficking of MHC class I molecules in Tapasin mutant mice. | Grandea, AG., et al. 2000. Immunity. 13: 213-22. PMID: 10981964
- Cloning and functional characterization of a subunit of the transporter associated with antigen processing. | Li, S., et al. 1997. Proc Natl Acad Sci U S A. 94: 8708-13. PMID: 9238042
- A critical role for tapasin in the assembly and function of multimeric MHC class I-TAP complexes. | Ortmann, B., et al. 1997. Science. 277: 1306-9. PMID: 9271576
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TPN Antibody (TO-3) | sc-80647 | 200 µg/ml | $322.00 | |||
TPN Antibody (TO-3): m-IgG Fc BP-HRP Bundle | sc-528696 | 200 µg Ab; 10 µg BP | $361.00 | |||
TPN Antibody (TO-3): m-IgGκ BP-HRP Bundle | sc-521157 | 200 µg Ab, 40 µg BP | $361.00 | |||
TPN Antibody (TO-3): m-IgG1 BP-HRP Bundle | sc-543069 | 200 µg Ab; 20 µg BP | $361.00 | |||
TPN Antibody (TO-3) AC | sc-80647 AC | 500 µg/ml, 25% agarose | $424.00 | |||
TPN Antibody (TO-3) HRP | sc-80647 HRP | 200 µg/ml | $322.00 | |||
TPN Antibody (TO-3) FITC | sc-80647 FITC | 200 µg/ml | $336.00 | |||
TPN Antibody (TO-3) PE | sc-80647 PE | 200 µg/ml | $349.00 | |||
TPN Antibody (TO-3) Alexa Fluor® 488 | sc-80647 AF488 | 200 µg/ml | $364.00 | |||
TPN Antibody (TO-3) Alexa Fluor® 546 | sc-80647 AF546 | 200 µg/ml | $364.00 | |||
TPN Antibody (TO-3) Alexa Fluor® 594 | sc-80647 AF594 | 200 µg/ml | $364.00 | |||
TPN Antibody (TO-3) Alexa Fluor® 647 | sc-80647 AF647 | 200 µg/ml | $364.00 | |||
TPN Antibody (TO-3) Alexa Fluor® 680 | sc-80647 AF680 | 200 µg/ml | $364.00 | |||
TPN Antibody (TO-3) Alexa Fluor® 790 | sc-80647 AF790 | 200 µg/ml | $364.00 |