TPN Antibody (F-6): sc-514852

TPN Antibody (F-6) is a mouse monoclonal IgG2a antibody that detects TPN in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TPN, also known as tapasin, is a type I membrane glycoprotein encoded by a gene located on chromosome 6p21, producing a 488-residue protein that plays a crucial role in the immune response. Specifically, TPN is essential for the proper assembly and function of major histocompatibility complex (MHC) class I molecules, as anti-TPN antibody (F-6) facilitates the loading of peptide antigens onto these molecules within the endoplasmic reticulum. The cytoplasmic domain of TPN contains a double-lysine motif that is believed to be critical for retention in the endoplasmic reticulum, ensuring that MHC class I molecules are properly assembled before being transported to the cell surface. Deficiencies in TPN can lead to impaired antigen presentation to CD8+ T cells, resulting in compromised immune responses, highlighting the importance of TPN in maintaining effective cellular immunity. TPN monoclonal antibody (F-6) is a valuable tool for researchers studying antigen processing and presentation mechanisms in human cells.

The preferred TPN antibody is TPN Antibody (TO-3): sc-80647.