TPN Antibody (B-9): sc-514853

TPN Antibody (B-9) is a mouse monoclonal IgG1 antibody that detects TPN in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TPN, also known as tapasin, is a type I membrane glycoprotein encoded by a gene located on chromosome 6p21, producing a 488-residue protein that plays a crucial role in the immune response. Specifically, TPN is essential for the proper assembly and function of major histocompatibility complex (MHC) class I molecules, as anti-TPN antibody (B-9) facilitates the transfer of peptide antigens onto these molecules within the endoplasmic reticulum. The cytoplasmic domain of TPN contains a double-lysine motif that is believed to be critical for retention in the endoplasmic reticulum, ensuring that MHC class I molecules are loaded with appropriate peptides before being transported to the cell surface. Notably, studies involving TPN knockout mice have demonstrated significant defects in the expression of MHC class I molecules on the cell surface, leading to impaired antigen presentation to CD8+ T cells and compromised humoral immune responses. This highlights the importance of TPN in maintaining effective immune surveillance and response, making TPN monoclonal antibody (B-9) a valuable tool for researchers studying immune mechanisms and related disorders.

The preferred TPN antibody is TPN Antibody (TO-3): sc-80647.