TPMT Antibody (E-8): sc-374154

TPMT Antibody (E-8) is a mouse monoclonal IgG2a kappa light chain antibody that detects TPMT protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TPMT (E-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Thiopurine S-methyltransferase (TPMT), also known as thiopurine methyltransferase, plays a crucial role in the metabolism of thiopurine drugs, such as 6-mercaptopurine, by catalyzing their S-methylation. This enzymatic activity determines therapeutic efficacy and potential toxicity of these medications, as variations in TPMT activity can lead to significant differences in drug response among individuals. TPMT is primarily located in the cytoplasm and typically exists as a monomer, although S-adenosyl-L-homocysteine can inhibit TPMT activity. Notably, TPMT activity varies across different ethnic groups due to genetic polymorphisms, with a significant proportion of Caucasians exhibiting high TPMT activity. Understanding TPMT function and regulation is essential for optimizing thiopurine therapy and minimizing adverse effects, making anti-TPMT antibody (E-8) a valuable tool for research and clinical applications.

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TPMT Antibody (E-8) References:

1. Whose TPMT activity is it anyway?  |  Ford, L., et al. 2004. Ann Clin Biochem. 41: 498-500. PMID: 15588444
2. Pharmacogenetics of inflammatory bowel disease.  |  Kelleher, D., et al. 2004. Novartis Found Symp. 263: 41-53; discussion 53-6, 211-8. PMID: 15669633
3. Cancer pharmacogenomics: powerful tools in cancer chemotherapy and drug development.  |  Lee, W., et al. 2005. Oncologist. 10: 104-11. PMID: 15709212
4. Thiopurine methyltransferase in acute lymphoblastic leukaemia: biochemical and molecular biological aspects.  |  Brouwer, C., et al. 2005. Eur J Cancer. 41: 613-23. PMID: 15737567
5. Measurement of thiopurine methyl transferase activity guides dose-initiation and prevents toxicity from azathioprine.  |  Sies, C., et al. 2005. N Z Med J. 118: U1324. PMID: 15776100
6. Thiopurine methyltransferase enzyme activity determination before treatment of inflammatory bowel disease with azathioprine: effect on cost and adverse events.  |  Sayani, FA., et al. 2005. Can J Gastroenterol. 19: 147-51. PMID: 15776134
7. Thiopurine methyltransferase (TPMT) genotype and early treatment response to mercaptopurine in childhood acute lymphoblastic leukemia.  |  Stanulla, M., et al. 2005. JAMA. 293: 1485-9. PMID: 15784872
8. Thiopurine methyltransferase (TPMT) heterozygosity and enzyme activity as predictive tests for the development of azathioprine-related adverse events.  |  Heckmann, JM., et al. 2005. J Neurol Sci. 231: 71-80. PMID: 15792824
9. Genotyping of thiopurine methyltransferase using pyrosequencing.  |  Okada, Y., et al. 2005. Biol Pharm Bull. 28: 677-81. PMID: 15802809
10. Interference free and simplyfied liquid chromatography-based determination of thiopurine S-methyltransferase activity in erythrocytes.  |  Khalil, MN., et al. 2005. J Chromatogr B Analyt Technol Biomed Life Sci. 821: 105-11. PMID: 15897017

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TPMT Antibody (E-8) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-TPMT Antibody (E-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TPMT Antibody (E-8) Specifications

Alternate Product Name: TPMT

Product Clone Name: E-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 38-115

Epitope Target: TPMT

Epitope Species: human

Product Reactivity/Detection: TPMT

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for TPMT Antibody (E-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin