TOPORS CRISPR/Cas9 KO Plasmid (h): sc-406762
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TOPORS CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TOPORS genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TOPORS Antibody (Y-30): sc-101182
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TOPORS CRISPR/Cas9 KO Plasmid (h) | sc-406762 | 20 µg | $397.00 |
Overview
TOPORS (topoisomerase I-binding, arginine/serine-rich protein) encodes a nuclear protein that functions as a RING-type E3 ubiquitin ligase and SUMO1 E3 ligase, integrating ubiquitination and sumoylation to control protein stability and signaling. It localizes to promyelocytic leukemia (PML) nuclear bodies and contributes to genome integrity through regulation of DNA damage response and transcription-associated processes. TOPORS has been linked to control of p53 signaling and proteostasis, connecting it to cellular stress responses and cell cycle regulation. Genetic variation and altered expression of TOPORS have been associated with retinal degeneration phenotypes and broader susceptibility to dysregulated nuclear signaling in disease-relevant contexts.
TOPORS CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TOPORS gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TOPORS together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TOPORS open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TOPORS protein expression.
This CRISPR knockout system enables efficient generation of TOPORS-deficient cell models for investigation of TOPORS signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TOPORS exon(s) critical for TOPORS function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TOPORS genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TOPORS CRISPR/Cas9 KO Plasmid (h) and TOPORS CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TOPORS locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TOPORS HDR Plasmid (h) and TOPORS HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TOPORS homology arms to support homology-directed repair at defined TOPORS target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.