TOPORS Antibody (Y-30): sc-101182

TOPORS Antibody (Y-30) is a mouse monoclonal IgG2a kappa light chain antibody that detects TOPORS protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-TOPORS antibody (Y-30) is available as the non-conjugated form. TOPORS protein, also known as topoisomerase I binding, arginine/serine-rich, LUN, RP31, P53BP3, or TP53BPL, is a 1,045 amino acid protein characterized by the presence of a RING-type zinc finger, which is crucial for function as a ubiquitin E3 ligase. TOPORS protein is primarily localized to discrete nuclear foci and is expressed in various tissues, with highest levels found in the testis. TOPORS protein′s ability to ubiquitinate key proteins, such as p53, plays a significant role in regulating cellular responses to DNA damage, as TOPORS targets these proteins for proteasomal degradation. Notably, TOPORS expression can be induced by genotoxic agents like camptothecin and cisplatin, highlighting importance in DNA damage repair pathways. Furthermore, decreased levels of TOPORS have been observed in lung and colon cancers, suggesting potential role as a tumor suppressor. Mutations in the gene encoding TOPORS are linked to retinitis pigmentosa type 31 (RP31), a degenerative condition that leads to progressive vision loss, underscoring TOPORS protein′s critical function in maintaining cellular integrity and vision.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

TOPORS Antibody (Y-30) References:

1. Interaction between human topoisomerase I and a novel RING finger/arginine-serine protein.  |  Haluska, P., et al. 1999. Nucleic Acids Res. 27: 2538-44. PMID: 10352183
2. Identification of a novel gene encoding a p53-associated protein.  |  Zhou, R., et al. 1999. Gene. 235: 93-101. PMID: 10415337
3. Cloning and characterization of LUN, a novel ring finger protein that is highly expressed in lung and specifically binds to a palindromic sequence.  |  Chu, D., et al. 2001. J Biol Chem. 276: 14004-13. PMID: 11278651
4. The topoisomerase I-binding RING protein, topors, is associated with promyelocytic leukemia nuclear bodies.  |  Rasheed, ZA., et al. 2002. Exp Cell Res. 277: 152-60. PMID: 12083797
5. The DNA topoisomerase I binding protein topors as a novel cellular target for SUMO-1 modification: characterization of domains necessary for subcellular localization and sumolation.  |  Weger, S., et al. 2003. Exp Cell Res. 290: 13-27. PMID: 14516784
6. The topoisomerase I- and p53-binding protein topors is differentially expressed in normal and malignant human tissues and may function as a tumor suppressor.  |  Saleem, A., et al. 2004. Oncogene. 23: 5293-300. PMID: 15107820
7. Topors functions as an E3 ubiquitin ligase with specific E2 enzymes and ubiquitinates p53.  |  Rajendra, R., et al. 2004. J Biol Chem. 279: 36440-4. PMID: 15247280
8. topors, a p53 and topoisomerase I-binding RING finger protein, is a coactivator of p53 in growth suppression induced by DNA damage.  |  Lin, L., et al. 2005. Oncogene. 24: 3385-96. PMID: 15735665
9. Mutations in TOPORS cause autosomal dominant retinitis pigmentosa with perivascular retinal pigment epithelium atrophy.  |  Chakarova, CF., et al. 2007. Am J Hum Genet. 81: 1098-103. PMID: 17924349
10. TOPORS-mediated RAD51 SUMOylation facilitates homologous recombination repair.  |  Hariharasudhan, G., et al. 2022. Nucleic Acids Res. 50: 1501-1516. PMID: 35061896
11. TOPORS as a novel causal gene for Joubert syndrome.  |  Strong, A., et al. 2023. Am J Med Genet A. 191: 2156-2163. PMID: 37227088
12. TOPORS E3 ligase mediates resistance to hypomethylating agent cytotoxicity in acute myeloid leukemia cells.  |  Truong, P., et al. 2024. Nat Commun. 15: 7360. PMID: 39198401

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TOPORS Antibody (Y-30) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-TOPORS Antibody (Y-30) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TOPORS Antibody (Y-30) Specifications

Alternate Product Name: TOPORS

Product Clone Name: Y-30

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 98-206

Epitope Target: TOPORS

Epitope Species: human

Product Reactivity/Detection: TOPORS

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for TOPORS Antibody (Y-30): N/A