TopBP1 Antibody (33): sc-136106
- TopBP1 Antibody (33) is a mouse monoclonal IgG1 provided at 50 µg/0.5 ml
- raised against amino acids 204-416 of TopBP1 of human origin
- recommended for detection of TopBP1 of human origin by WB, IP and IF
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for TopBP1 Antibody (33). This work is in progress.
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TopBP1 Antibody (33) is a mouse monoclonal IgG1 antibody that detects TopBP1 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). TopBP1 is a crucial protein that plays a significant role in the DNA damage response, transcription, and replication processes, containing eight BRCT motifs that are characteristic of proteins involved in these critical cellular functions. Anti-TopBP1 antibody (33) recognizes nuclear foci and localizes to sites of DNA damage or stalled replication forks, underscoring TopBP1′s importance in maintaining genomic stability. Notably, TopBP1 is phosphorylated in response to DNA double-strand breaks and replication blocks, which is vital for function in the DNA damage response pathway. Furthermore, TopBP1 interacts specifically with the C-terminal region of topoisomerase II beta, suggesting facilitation of catalytic reactions by promoting transient breakages of DNA strands. The gene encoding TopBP1 is located on chromosome 3q22.2, and downregulation has been associated with reduced cell survival, likely due to increased apoptosis, highlighting TopBP1′s essential role in cellular health and response to DNA damage.
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TopBP1 Antibody (33) References:
- TopBP1 and DNA polymerase alpha-mediated recruitment of the 9-1-1 complex to stalled replication forks: implications for a replication restart-based mechanism for ATR checkpoint activation. | Yan, S. and Michael, WM. 2009. Cell Cycle. 8: 2877-84. PMID: 19652550
- Functions of TopBP1 in preserving genome integrity during mitosis. | Bagge, J., et al. 2021. Semin Cell Dev Biol. 113: 57-64. PMID: 32912640
- CK2 kinase-mediated PHF8 phosphorylation controls TopBP1 stability to regulate DNA replication. | Feng, H., et al. 2020. Nucleic Acids Res. 48: 10940-10952. PMID: 33010150
- TopBP1 assembles nuclear condensates to switch on ATR signaling. | Frattini, C., et al. 2021. Mol Cell. 81: 1231-1245.e8. PMID: 33503405
- Overexpression of TopBP1, a canonical ATR/Chk1 activator, paradoxically hinders ATR/Chk1 activation in cancer. | Liu, K., et al. 2021. J Biol Chem. 296: 100382. PMID: 33556369
- The CIP2A-TOPBP1 axis safeguards chromosome stability and is a synthetic lethal target for BRCA-mutated cancer. | Adam, S., et al. 2021. Nat Cancer. 2: 1357-1371. PMID: 35121901
- A small-molecule inhibitor of TopBP1 exerts anti-MYC activity and synergy with PARP inhibitors. | Lin, FT., et al. 2023. Proc Natl Acad Sci U S A. 120: e2307793120. PMID: 37878724
- NRF2 promotes radiation resistance by cooperating with TOPBP1 to activate the ATR-CHK1 signaling pathway. | Sun, X., et al. 2024. Theranostics. 14: 681-698. PMID: 38169561
- Spatial organization and functions of Chk1 activation by TopBP1 biomolecular condensates. | Egger, T., et al. 2024. Cell Rep. 43: 114064. PMID: 38578830
- A human papillomavirus 16 E2-TopBP1 dependent SIRT1-p300 acetylation switch regulates mitotic viral and human protein levels and activates the DNA damage response. | Prabhakar, AT., et al. 2024. mBio. 15: e0067624. PMID: 38722185
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TopBP1 Antibody (33) | sc-136106 | 50 µg/0.5 ml | $316.00 |