Tom70 Antibody (A-8): sc-390545

Tom70 Antibody (A-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects Tom70 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Tom70 (A-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Tom70 protein plays a crucial role in mitochondrial protein translocation, acting as a receptor that recognizes preproteins destined for mitochondria. This process ensures proteins necessary for mitochondrial function, such as those involved in energy production and apoptosis, are properly imported into the organelle. Tom70 protein is part of the mitochondrial protein translocase complex, which includes several integral membrane proteins that facilitate preprotein transport across mitochondrial membranes. The human Tom70 gene is located on chromosome 3q12.2, highlighting its significance in human health and disease. With proven detection of Tom70 across multiple species and applications, anti-Tom70 antibody (A-8) serves as an essential tool for researchers studying mitochondrial dynamics and function.

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Tom70 Antibody (A-8) References:

1. An internal targeting signal directing proteins into the mitochondrial intermembrane space.  |  Diekert, K., et al. 1999. Proc Natl Acad Sci U S A. 96: 11752-7. PMID: 10518522
2. Protein translocation across membranes.  |  Agarraberes, FA. and Dice, JF. 2001. Biochim Biophys Acta. 1513: 1-24. PMID: 11427190
3. Characterization of rat TOM70 as a receptor of the preprotein translocase of the mitochondrial outer membrane.  |  Suzuki, H., et al. 2002. J Cell Sci. 115: 1895-905. PMID: 11956321
4. Molecular chaperones Hsp90 and Hsp70 deliver preproteins to the mitochondrial import receptor Tom70.  |  Young, JC., et al. 2003. Cell. 112: 41-50. PMID: 12526792
5. Isolation and characterization of an IgNAR variable domain specific for the human mitochondrial translocase receptor Tom70.  |  Nuttall, SD., et al. 2003. Eur J Biochem. 270: 3543-54. PMID: 12919318
6. A biophysical analysis of the tetratricopeptide repeat-rich mitochondrial import receptor, Tom70, reveals an elongated monomer that is inherently flexible, unstable, and unfolds via a multistate pathway.  |  Beddoe, T., et al. 2004. J Biol Chem. 279: 46448-54. PMID: 15316022
7. Tom70 protects against diabetic cardiomyopathy through its antioxidant and antiapoptotic properties.  |  Wang, P., et al. 2020. Hypertens Res. 43: 1047-1056. PMID: 32724135
8. The Mitochondrial Outer Membrane Protein Tom70-Mediator in Protein Traffic, Membrane Contact Sites and Innate Immunity.  |  Kreimendahl, S. and Rassow, J. 2020. Int J Mol Sci. 21: PMID: 33019591
9. A cold-stress-inducible PERK/OGT axis controls TOM70-assisted mitochondrial protein import and cristae formation.  |  Latorre-Muro, P., et al. 2021. Cell Metab. 33: 598-614.e7. PMID: 33592173
10. Tom70-based transcriptional regulation of mitochondrial biogenesis and aging.  |  Liu, Q., et al. 2022. Elife. 11: PMID: 35234609
11. The Tim core complex defines the number of mitochondrial translocation contact sites and can hold arrested preproteins in the absence of matrix Hsp70-Tim44.  |  Dekker, PJ., et al. 1997. EMBO J. 16: 5408-19. PMID: 9312000
12. Tom40 forms the hydrophilic channel of the mitochondrial import pore for preproteins [see comment].  |  Hill, K., et al. 1998. Nature. 395: 516-21. PMID: 9774109

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Tom70 Antibody (A-8) has 39 citations in a variety of scientific publications.

Primary antibodies like Anti-Tom70 Antibody (A-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Tom70 Antibody (A-8) Specifications

Alternate Product Name: Tom70

Product Clone Name: A-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 492-608

Epitope Target: Tom70

Epitope Species: human

Product Reactivity/Detection: Tom70

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine and canine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 39 citations in various scientific publications

Available Conjugates/Forms for Tom70 Antibody (A-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin