Tom1 Antibody (H-5): sc-514430
- Tom1 Antibody (H-5) is a mouse monoclonal IgG2a κ Tom1 antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 368-439 mapping near the C-terminus of Tom1 of human origin
- Tom1 Antibody (H-5) is recommended for detection of Tom1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Tom1 Antibody (H-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Tom1 Antibody (H-5) for WB applications. These reagents are now offered in bundles with Tom1 Antibody (H-5) (see ordering information below).
Tom1 Antibody (H-5) is a mouse monoclonal IgG2a kappa light chain antibody that detects Tom1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Tom1 (H-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Tom1 protein, also known as Target of Myb 1, is a 492 amino acid cytoplasmic protein that plays a crucial role in intracellular trafficking, particularly in moving ubiquitinated proteins to early endosomes for degradation. This process maintains cellular homeostasis and regulates protein turnover, impacting various cellular functions and signaling pathways. Tom1 features one GAT domain and one VHS domain, which enable interactions with other proteins, such as Tollip and endofin (SARA). These interactions help recruit Tom1 to the endosome, enabling participation in endosomal trafficking of ubiquitin-tagged proteins. Human Tom1 shows 76% similarity with chicken Tom1 and 89% similarity with mouse Tom1, indicating a conserved function across species that highlights its importance in cellular processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Tom1 Antibody (H-5) References:
- TOM1 genes map to human chromosome 22q13.1 and mouse chromosome 8C1 and encode proteins similar to the endosomal proteins HGS and STAM. | Seroussi, E., et al. 1999. Genomics. 57: 380-8. PMID: 10329004
- Structure of the VHS domain of human Tom1 (target of myb 1): insights into interactions with proteins and membranes. | Misra, S., et al. 2000. Biochemistry. 39: 11282-90. PMID: 10985773
- Tom1, a VHS domain-containing protein, interacts with tollip, ubiquitin, and clathrin. | Yamakami, M., et al. 2003. J Biol Chem. 278: 52865-72. PMID: 14563850
- Endofin recruits TOM1 to endosomes. | Seet, LF., et al. 2004. J Biol Chem. 279: 4670-9. PMID: 14613930
- Tollip and Tom1 form a complex and recruit ubiquitin-conjugated proteins onto early endosomes. | Katoh, Y., et al. 2004. J Biol Chem. 279: 24435-43. PMID: 15047686
- Endofin recruits clathrin to early endosomes via TOM1. | Seet, LF. and Hong, W. 2005. J Cell Sci. 118: 575-87. PMID: 15657082
- Structural basis for recognition of ubiquitinated cargo by Tom1-GAT domain. | Akutsu, M., et al. 2005. FEBS Lett. 579: 5385-91. PMID: 16199040
- Recruitment of clathrin onto endosomes by the Tom1-Tollip complex. | Katoh, Y., et al. 2006. Biochem Biophys Res Commun. 341: 143-9. PMID: 16412388
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Tom1 Antibody (H-5) | sc-514430 | 200 µg/ml | $322.00 | |||
Tom1 Antibody (H-5): m-IgG Fc BP-HRP Bundle | sc-531124 | 200 µg Ab; 10 µg BP | $361.00 | |||
Tom1 Antibody (H-5): m-IgGκ BP-HRP Bundle | sc-524723 | 200 µg Ab, 40 µg BP | $361.00 | |||
Tom1 Antibody (H-5): m-IgG2a BP-HRP Bundle | sc-547693 | 200 µg Ab; 10 µg BP | $361.00 | |||
Tom1 Antibody (H-5) AC | sc-514430 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Tom1 Antibody (H-5) HRP | sc-514430 HRP | 200 µg/ml | $322.00 | |||
Tom1 Antibody (H-5) FITC | sc-514430 FITC | 200 µg/ml | $336.00 | |||
Tom1 Antibody (H-5) PE | sc-514430 PE | 200 µg/ml | $349.00 | |||
Tom1 Antibody (H-5) Alexa Fluor® 488 | sc-514430 AF488 | 200 µg/ml | $364.00 | |||
Tom1 Antibody (H-5) Alexa Fluor® 546 | sc-514430 AF546 | 200 µg/ml | $364.00 | |||
Tom1 Antibody (H-5) Alexa Fluor® 594 | sc-514430 AF594 | 200 µg/ml | $364.00 | |||
Tom1 Antibody (H-5) Alexa Fluor® 647 | sc-514430 AF647 | 200 µg/ml | $364.00 | |||
Tom1 Antibody (H-5) Alexa Fluor® 680 | sc-514430 AF680 | 200 µg/ml | $364.00 | |||
Tom1 Antibody (H-5) Alexa Fluor® 790 | sc-514430 AF790 | 200 µg/ml | $364.00 |