TOB1 CRISPR/Cas9 KO Plasmid (m): sc-423508
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TOB1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TOB1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TOB1 Antibody (E-1): sc-133095
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TOB1 CRISPR/Cas9 KO Plasmid (m) | sc-423508 | 20 µg | $397.00 |
Overview
Tob1 encodes TOB1, a member of the antiproliferative BTG/TOB family that functions as an immediate-early transcriptional coregulator and modulates cell-cycle progression. TOB1 is regulated by MAPK-dependent phosphorylation and interfaces with SMAD transcriptional programs to shape TGF-β signaling outputs, including effects on differentiation and growth restraint. In immune cells, TOB1 contributes to maintenance of activation thresholds and tolerance by dampening T cell activation and cytokine production, linking it to pathways controlling immune homeostasis. Altered TOB1 activity has been associated with dysregulated proliferation and immune-related phenotypes, making it relevant for mechanistic studies of oncogenic signaling, inflammation, and tissue remodeling in mouse models.
TOB1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Tob1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Tob1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Tob1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TOB1 protein expression.
This CRISPR knockout system enables efficient generation of Tob1-deficient cell models for investigation of TOB1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Tob1 exon(s) critical for TOB1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Tob1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TOB1 CRISPR/Cas9 KO Plasmid (m) and TOB1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Tob1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TOB1 HDR Plasmid (m) and TOB1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Tob1 homology arms to support homology-directed repair at defined Tob1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.