TNP2 CRISPR/Cas9 KO Plasmid (m): sc-423464
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TNP2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TNP2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TNP2 Antibody (B-2): sc-393843
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TNP2 CRISPR/Cas9 KO Plasmid (m) | sc-423464 | 20 µg | $397.00 |
Overview
Tnp2 encodes transition protein 2 (TNP2), a sperm-specific chromatin remodeling factor that replaces histones during late spermiogenesis and facilitates the subsequent incorporation of protamines for stable DNA compaction. This protein participates in the coordinated histone-to-protamine exchange program, supporting nuclear condensation, genome integrity, and proper sperm head morphology. Disruption of Tnp2 perturbs chromatin packaging dynamics and can lead to impaired sperm maturation and reduced male fertility phenotypes. As a model, mouse TNP2 is widely used to study epigenetic reprogramming, post-meiotic transcriptional shutdown, and the regulation of chromatin architecture in germ cells.
TNP2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Tnp2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Tnp2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Tnp2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TNP2 protein expression.
This CRISPR knockout system enables efficient generation of Tnp2-deficient cell models for investigation of TNP2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Tnp2 exon(s) critical for TNP2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Tnp2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TNP2 CRISPR/Cas9 KO Plasmid (m) and TNP2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Tnp2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TNP2 HDR Plasmid (m) and TNP2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Tnp2 homology arms to support homology-directed repair at defined Tnp2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.