TNIK Antibody (53): sc-136103

TNIK Antibody (53) is a mouse monoclonal IgG1 kappa light chain antibody that detects TNIK in mouse, rat, and human samples through western blotting (WB) and immunoprecipitation (IP). Anti-TNIK antibody (53) is available in a non-conjugated form, providing researchers with a versatile tool for studying TNIK′s role in various biological processes. TNIK, or TRAF2 and NCK-interacting protein kinase, is a significant 1,360 amino acid protein that plays a crucial role in cellular signaling pathways, particularly in response to environmental stress. TNIK contains a protein kinase domain and is classified within the serine/threonine protein kinase family, which is essential for the phosphorylation and dephosphorylation of proteins on serine and threonine residues. This post-translational modification is vital for regulating cellular functions, including cell division, apoptosis, and maintaining cellular homeostasis. TNIK is expressed ubiquitously, with highest levels found in the brain, heart, and skeletal muscle, indicating importance in both neuronal and muscular functions. TNIK exists in eight isoforms generated through alternative splicing, which may contribute to diverse functional roles in different tissues. TNIK monoclonal antibody (53) enables researchers to gain insights into mechanisms by which TNIK influences cellular responses to stress and potential implications in various diseases.

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TNIK Antibody (53) References:

1. TNIK, a novel member of the germinal center kinase family that activates the c-Jun N-terminal kinase pathway and regulates the cytoskeleton.  |  Fu, CA., et al. 1999. J Biol Chem. 274: 30729-37. PMID: 10521462
2. Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry.  |  Brill, LM., et al. 2004. Anal Chem. 76: 2763-72. PMID: 15144186
3. The Traf2- and Nck-interacting kinase as a putative effector of Rap2 to regulate actin cytoskeleton.  |  Taira, K., et al. 2004. J Biol Chem. 279: 49488-96. PMID: 15342639
4. Proteomics analysis of protein kinases by target class-selective prefractionation and tandem mass spectrometry.  |  Wissing, J., et al. 2007. Mol Cell Proteomics. 6: 537-47. PMID: 17192257
5. Protein kinase classification.  |  Hunter, T. 1991. Methods Enzymol. 200: 3-37. PMID: 1835513
6. The protein kinase family: conserved features and deduced phylogeny of the catalytic domains.  |  Hanks, SK., et al. 1988. Science. 241: 42-52. PMID: 3291115
7. TNIK is a conserved regulator of glucose and lipid metabolism in obesity.  |  Pham, TCP., et al. 2023. Sci Adv. 9: eadf7119. PMID: 37556547
8. TNIK drives castration-resistant prostate cancer via phosphorylating EGFR.  |  Guo, J., et al. 2024. iScience. 27: 108713. PMID: 38226156
9. A small-molecule TNIK inhibitor targets fibrosis in preclinical and clinical models.  |  Ren, F., et al. 2025. Nat Biotechnol. 43: 63-75. PMID: 38459338
10. TNIK depletion induces inflammation and apoptosis in injured renal proximal tubule epithelial cells.  |  Bradford, STJ., et al. 2024. Am J Physiol Renal Physiol. 326: F827-F838. PMID: 38482555
11. TNIK's emerging role in cancer, metabolism, and age-related diseases.  |  Ewald, CY., et al. 2024. Trends Pharmacol Sci. 45: 478-489. PMID: 38777670
12. Prediction of the coding sequences of unidentified human genes. IX. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro.  |  Nagase, T., et al. 1998. DNA Res. 5: 31-9. PMID: 9628581

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TNIK Antibody (53) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-TNIK Antibody (53) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TNIK Antibody (53) Specifications

Alternate Product Name: TNIK

Product Clone Name: 53

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 522-644

Epitope Target: TNIK

Epitope Species: human

Product Reactivity/Detection: TNIK

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for TNIK Antibody (53): N/A

The preferred TNIK antibody is TNIK Antibody (C-1): sc-377215.