TNFβ Antibody (E-6): sc-28345
- TNFβ Antibody (E-6) is a mouse monoclonal IgG1 κ TNFβ antibody, cited in 5 publications, provided at 200 µg/ml
- raised against amino acids 35-205 of TNFβ of human origin
- TNF beta Antibody (E-6) is recommended for detection of TNFβ of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-TNF beta Antibody (E-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for TNFβ Antibody (E-6) for WB applications. These reagents are now offered in bundles with TNFβ Antibody (E-6) (see ordering information below).
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TNFβ Antibody (E-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects TNFβ in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TNFβ (E-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Tumor necrosis factor beta (TNFβ), also known as lymphotoxin, is a pleiotropic cytokine that plays a crucial role in immune response and inflammation. TNFβ is primarily produced by activated lymphocytes, including CD4(+) T helper type 1 cells, CD8(+) lymphocytes, and certain B lymphoblastoid cell lines. TNFβ is essential for mediating various immune functions, including apoptosis regulation and immune cell activation, which is vital for maintaining homeostasis and combating infections. TNFβ shares approximately 30% amino acid homology with tumor necrosis factor alpha (TNFα), another important cytokine that exerts similar biological effects. TNFα and TNFβ can modulate immune and inflammatory responses while possessing the ability to inhibit tumor growth, with target tumor cells requiring TNF receptors 1 and 2 for effective cytotoxicity. p55 receptor is particularly significant in mediating cytotoxic response, highlighting TNFβ′s importance in therapeutic strategies against cancer and autoimmune diseases.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
TNFβ Antibody (E-6) References:
- TNF-alpha and TNF-beta gene polymorphisms in systemic sclerosis. | Pandey, JP. and Takeuchi, F. 1999. Hum Immunol. 60: 1128-30. PMID: 10600011
- The modulation of cytokine and IgE production by tumor necrosis factor-beta in atopic dermatitis. | Jirapongsananuruk, O., et al. 2000. J Invest Dermatol. 114: 200-3. PMID: 10620139
- Tumor necrosis factor. New insights into the molecular mechanisms of its multiple actions. | Vilcek, J. and Lee, TH. 1991. J Biol Chem. 266: 7313-6. PMID: 1850405
- Tumor necrosis factor beta (TNF-beta) induces binding of the NF-kappa B transcription factor to a high-affinity kappa B element in the TNF-beta promoter. | Messer, G., et al. 1990. Cytokine. 2: 389-97. PMID: 2104232
- Human lymphotoxin and tumor necrosis factor genes: structure, homology and chromosomal localization. | Nedwin, GE., et al. 1985. Nucleic Acids Res. 13: 6361-73. PMID: 2995927
- Human tumor necrosis factor. Production, purification, and characterization. | Aggarwal, BB., et al. 1985. J Biol Chem. 260: 2345-54. PMID: 3871770
- Cytotoxic effect of TNF and lymphotoxin on T lymphoblasts. | Sarin, A., et al. 1995. J Immunol. 155: 3716-8. PMID: 7561073
- Tumor growth inhibition mediated by lymphotoxin: evidence of B lymphocyte involvement in the antitumor response. | Qin, Z. and Blankenstein, T. 1995. Cancer Res. 55: 4747-51. PMID: 7585497
- Abnormal development of peripheral lymphoid organs in mice deficient in lymphotoxin. | De Togni, P., et al. 1994. Science. 264: 703-7. PMID: 8171322
- Tumor necrosis factor's cytotoxic activity is signaled by the p55 TNF receptor. | Tartaglia, LA., et al. 1993. Cell. 73: 213-6. PMID: 8386591
- Lymphotoxin-alpha (TNF-beta) during sepsis. | Sriskandan, S., et al. 1996. Cytokine. 8: 933-7. PMID: 9050752
- Tumor necrosis factor-beta in human pregnancy and labor. | Laham, N., et al. 1997. J Reprod Immunol. 33: 53-69. PMID: 9185077
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TNFβ Antibody (E-6) | sc-28345 | 200 µg/ml | $322.00 | |||
TNFβ Antibody (E-6): m-IgG Fc BP-HRP Bundle | sc-528428 | 200 µg Ab; 10 µg BP | $361.00 | |||
TNFβ Antibody (E-6): m-IgGκ BP-HRP Bundle | sc-520803 | 200 µg Ab, 40 µg BP | $361.00 | |||
TNFβ Antibody (E-6): m-IgG1 BP-HRP Bundle | sc-542928 | 200 µg Ab; 20 µg BP | $361.00 | |||
TNFβ Antibody (E-6) AC | sc-28345 AC | 500 µg/ml, 25% agarose | $424.00 | |||
TNFβ Antibody (E-6) HRP | sc-28345 HRP | 200 µg/ml | $322.00 | |||
TNFβ Antibody (E-6) FITC | sc-28345 FITC | 200 µg/ml | $336.00 | |||
TNFβ Antibody (E-6) PE | sc-28345 PE | 200 µg/ml | $349.00 | |||
TNFβ Antibody (E-6) Alexa Fluor® 488 | sc-28345 AF488 | 200 µg/ml | $364.00 | |||
TNFβ Antibody (E-6) Alexa Fluor® 546 | sc-28345 AF546 | 200 µg/ml | $364.00 | |||
TNFβ Antibody (E-6) Alexa Fluor® 594 | sc-28345 AF594 | 200 µg/ml | $364.00 | |||
TNFβ Antibody (E-6) Alexa Fluor® 647 | sc-28345 AF647 | 200 µg/ml | $364.00 | |||
TNFβ Antibody (E-6) Alexa Fluor® 680 | sc-28345 AF680 | 200 µg/ml | $364.00 | |||
TNFβ Antibody (E-6) Alexa Fluor® 790 | sc-28345 AF790 | 200 µg/ml | $364.00 |