TMP21 Antibody (A-7): sc-137003
- TMP21 Antibody (A-7) is a mouse monoclonal IgG2a κ TMP21 antibody, cited in 4 publications, provided at 200 µg/ml
- raised against amino acids 41-105 mapping near the N-terminus of TMP21 of human origin
- TMP21 Antibody (A-7) is recommended for detection of TMP21 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-TMP21 Antibody (A-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for TMP21 Antibody (A-7) for WB and IHC(P) applications. These reagents are now offered in bundles with TMP21 Antibody (A-7) (see ordering information below).
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TMP21 Antibody (A-7) is a mouse monoclonal IgG2a kappa light chain antibody that detects TMP21 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-TMP21 antibody (A-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TMP21, also known as the 21 kDa transmembrane-trafficking protein or TMED10, is a crucial component of the EMP24/GP25L/p24 cargo family, which plays a significant role in regulating vesicular trafficking within the early secretory pathway. TMP21 is predominantly localized to the Golgi cisternae and the plasma membrane, where TMP21 functions as a single-pass type I membrane protein. A GOLD (Golgi dynamics) domain in TMP21 is essential for involvement in protein transport and quality control between the endoplasmic reticulum and the Golgi complex, ensuring that only properly folded and assembled proteins proceed through the secretory pathway. TMP21 is a vital component of the heteromeric secretase complex, also known as the Presenilin complex, where TMP21 modulates γ-secretase activity, a process that is critical for the regulation of various signaling pathways and maintenance of cellular homeostasis. Two isoforms, TMP21-I and TMP21-II, further highlight the complexity and functional diversity of TMP21, making TMP21 (A-7) monoclonal antibody an invaluable tool for researchers studying protein trafficking and processing in cellular biology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
TMP21 Antibody (A-7) References:
- A comparative study of rat and human Tmp21 (p23) reveals the pseudogene-like features of human Tmp21-II. | Hörer, J., et al. 1999. DNA Seq. 10: 121-6. PMID: 10376215
- Tmp21-I, a vesicular trafficking protein, is differentially expressed during induction of the ureter and metanephros. | Baker, LA. and Gomez, RA. 2000. J Urol. 164: 562-6. PMID: 10893644
- Chimaerins, novel non-protein kinase C phorbol ester receptors, associate with Tmp21-I (p23): evidence for a novel anchoring mechanism involving the chimaerin C1 domain. | Wang, H. and Kazanietz, MG. 2002. J Biol Chem. 277: 4541-50. PMID: 11689559
- Golgi matrix proteins interact with p24 cargo receptors and aid their efficient retention in the Golgi apparatus. | Barr, FA., et al. 2001. J Cell Biol. 155: 885-91. PMID: 11739402
- TMP21 is a presenilin complex component that modulates gamma-secretase but not epsilon-secretase activity. | Chen, F., et al. 2006. Nature. 440: 1208-12. PMID: 16641999
- Dual roles of the transmembrane protein p23/TMP21 in the modulation of amyloid precursor protein metabolism. | Vetrivel, KS., et al. 2007. Mol Neurodegener. 2: 4. PMID: 17288597
- Cloning of a gene bearing missense mutations in early-onset familial Alzheimer's disease. | Sherrington, R., et al. 1995. Nature. 375: 754-60. PMID: 7596406
- Tmp21 and p24A, two type I proteins enriched in pancreatic microsomal membranes, are members of a protein family involved in vesicular trafficking. | Blum, R., et al. 1996. J Biol Chem. 271: 17183-9. PMID: 8663407
- Intracellular localization and in vivo trafficking of p24A and p23. | Blum, R., et al. 1999. J Cell Sci. 112 (Pt 4): 537-48. PMID: 9914165
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TMP21 Antibody (A-7) | sc-137003 | 200 µg/ml | $316.00 | |||
TMP21 Antibody (A-7): m-IgGκ BP-HRP Bundle | sc-521390 | 200 µg Ab, 40 µg BP | $354.00 | |||
TMP21 Antibody (A-7): m-IgG2a BP-HRP Bundle | sc-546094 | 200 µg Ab; 10 µg BP | $354.00 | |||
TMP21 Antibody (A-7) AC | sc-137003 AC | 500 µg/ml, 25% agarose | $416.00 | |||
TMP21 Antibody (A-7) HRP | sc-137003 HRP | 200 µg/ml | $316.00 | |||
TMP21 Antibody (A-7) FITC | sc-137003 FITC | 200 µg/ml | $330.00 | |||
TMP21 Antibody (A-7) PE | sc-137003 PE | 200 µg/ml | $343.00 | |||
TMP21 Antibody (A-7) Alexa Fluor® 488 | sc-137003 AF488 | 200 µg/ml | $357.00 | |||
TMP21 Antibody (A-7) Alexa Fluor® 546 | sc-137003 AF546 | 200 µg/ml | $357.00 | |||
TMP21 Antibody (A-7) Alexa Fluor® 594 | sc-137003 AF594 | 200 µg/ml | $357.00 | |||
TMP21 Antibody (A-7) Alexa Fluor® 647 | sc-137003 AF647 | 200 µg/ml | $357.00 | |||
TMP21 Antibody (A-7) Alexa Fluor® 680 | sc-137003 AF680 | 200 µg/ml | $357.00 | |||
TMP21 Antibody (A-7) Alexa Fluor® 790 | sc-137003 AF790 | 200 µg/ml | $357.00 |