TLX2 CRISPR/Cas9 KO Plasmid (m): sc-423424
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TLX2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TLX2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TLX2 Antibody (D-12): sc-514077
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TLX2 CRISPR/Cas9 KO Plasmid (m) | sc-423424 | 20 µg | $397.00 |
Overview
Tlx2 encodes the homeobox transcription factor TLX2 (also known as HOX11L1), a nuclear regulator implicated in neuronal lineage specification and maturation during development. TLX2 influences gene expression programs that coordinate differentiation, migration, and connectivity within the peripheral and central nervous systems, interfacing with broader homeobox-controlled developmental networks. In mouse models, altered TLX2 activity has been associated with abnormalities in enteric nervous system formation and autonomic neuronal development, linking it to pathways governing gut motility and neurodevelopmental phenotypes. These features make TLX2 a useful node for studying transcriptional control of neural development and downstream effector circuits.
TLX2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Tlx2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Tlx2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Tlx2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TLX2 protein expression.
This CRISPR knockout system enables efficient generation of Tlx2-deficient cell models for investigation of TLX2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Tlx2 exon(s) critical for TLX2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Tlx2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TLX2 CRISPR/Cas9 KO Plasmid (m) and TLX2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Tlx2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TLX2 HDR Plasmid (m) and TLX2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Tlx2 homology arms to support homology-directed repair at defined Tlx2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.