TLX2 Antibody (D-12): sc-514077
- TLX2 Antibody (D-12) is a mouse monoclonal IgG1 κ TLX2 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 1-17 at the N-terminus of TLX2 of human origin
- TLX2 Antibody (D-12) is recommended for detection of TLX2 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-TLX2 Antibody (D-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-514077 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for TLX2 Antibody (D-12) for WB applications. These reagents are now offered in bundles with TLX2 Antibody (D-12) (see ordering information below).
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TLX2 Antibody (D-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects TLX2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TLX2 (D-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. T-cell leukemia homeobox protein 2 (TLX2), also known as homeobox protein Hox-11L1, neural crest homeobox protein, or ENX, is a 284 amino acid member of the TLX homeobox family, which plays a critical role in the development of the enteric nervous system. TLX2 is primarily localized in the nucleus, where TLX2 is involved in regulating gene expression essential for the proliferation and differentiation of neural crest-derived tissues. Notably, TLX2′s expression is restricted to tissues derived from neural crest cells, indicating TLX2′s importance in the development of the peripheral nervous system. Mutations in the gene encoding TLX2 have been linked to congenital anomalies resembling neuronal intestinal dysplasia in humans, a rare condition characterized by hyperplasia of the submucosal plexus and increased acetylcholinesterase activity in nerve fibers surrounding submucosal blood vessels. This highlights TLX2′s significance in both normal development and disease pathology, making anti-TLX2 antibody (D-12) a valuable tool for researchers studying neural crest development and related disorders.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
TLX2 Antibody (D-12) References:
- An enhancer element for expression of the Ncx (Enx, Hox11L1) gene in neural crest-derived cells. | Iitsuka, Y., et al. 1999. J Biol Chem. 274: 24401-7. PMID: 10446220
- Allelic loss of Hox11L1 gene locus predicts outcome of gastrointestinal stromal tumors. | Kaifi, JT., et al. 2006. Oncol Rep. 16: 915-9. PMID: 16969514
- Transcriptional regulation of TLX2 and impaired intestinal innervation: possible role of the PHOX2A and PHOX2B genes. | Borghini, S., et al. 2007. Eur J Hum Genet. 15: 848-55. PMID: 17505528
- The mechanism of intestinal motility in homozygous mutant Ncx/Hox11L.1-deficient mice--a model for intestinal neuronal dysplasia. | Kobayashi, H., et al. 2007. J Pediatr Surg. 42: 2062-6. PMID: 18082708
- Novel homeobox genes are differentially expressed in placental microvascular endothelial cells compared with macrovascular cells. | Murthi, P., et al. 2008. Placenta. 29: 624-30. PMID: 18514308
- The HOX11 gene encodes a DNA-binding nuclear transcription factor belonging to a distinct family of homeobox genes. | Dear, TN., et al. 1993. Proc Natl Acad Sci U S A. 90: 4431-5. PMID: 8099440
- Ncx, a Hox11 related gene, is expressed in a variety of tissues derived from neural crest cells. | Hatano, M., et al. 1997. Anat Embryol (Berl). 195: 419-25. PMID: 9176664
- A novel pathogenesis of megacolon in Ncx/Hox11L.1 deficient mice. | Hatano, M., et al. 1997. J Clin Invest. 100: 795-801. PMID: 9259577
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TLX2 Antibody (D-12) | sc-514077 | 200 µg/ml | $316.00 | |||
TLX2 Antibody (D-12): m-IgG Fc BP-HRP Bundle | sc-527849 | 200 µg Ab; 10 µg BP | $354.00 | |||
TLX2 Antibody (D-12): m-IgG1 BP-HRP Bundle | sc-533222 | 200 µg Ab; 20 µg BP | $354.00 | |||
TLX2 Antibody (D-12) X | sc-514077 X | 200 µg/0.1 ml | $316.00 | |||
TLX2 Antibody (D-12) AC | sc-514077 AC | 500 µg/ml, 25% agarose | $416.00 | |||
TLX2 Antibody (D-12) HRP | sc-514077 HRP | 200 µg/ml | $316.00 | |||
TLX2 Antibody (D-12) FITC | sc-514077 FITC | 200 µg/ml | $330.00 | |||
TLX2 Antibody (D-12) PE | sc-514077 PE | 200 µg/ml | $343.00 | |||
TLX2 Antibody (D-12) Alexa Fluor® 488 | sc-514077 AF488 | 200 µg/ml | $357.00 | |||
TLX2 Antibody (D-12) Alexa Fluor® 546 | sc-514077 AF546 | 200 µg/ml | $357.00 | |||
TLX2 Antibody (D-12) Alexa Fluor® 594 | sc-514077 AF594 | 200 µg/ml | $357.00 | |||
TLX2 Antibody (D-12) Alexa Fluor® 647 | sc-514077 AF647 | 200 µg/ml | $357.00 | |||
TLX2 Antibody (D-12) Alexa Fluor® 680 | sc-514077 AF680 | 200 µg/ml | $357.00 | |||
TLX2 Antibody (D-12) Alexa Fluor® 790 | sc-514077 AF790 | 200 µg/ml | $357.00 | |||
TLX2 (D-12) Neutralizing Peptide | sc-514077 P | 100 µg/0.5 ml | $68.00 |