TLR8 CRISPR/Cas9 KO Plasmid (m): sc-431303
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TLR8 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TLR8 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TLR8 Antibody (D-8): sc-373760
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TLR8 CRISPR/Cas9 KO Plasmid (m) | sc-431303 | 20 µg | $397.00 |
Overview
Tlr8 encodes Toll-like receptor 8 (TLR8), an endosomal pattern-recognition receptor that detects pathogen-derived single-stranded RNA and select synthetic agonists to initiate innate immune signaling. Upon activation, TLR8 engages MYD88-dependent cascades that converge on NF-κB and MAPK pathways, promoting transcriptional programs for inflammatory cytokines and co-stimulatory mediators that shape myeloid and lymphoid responses. In mouse, TLR8 contributes to regulation of cytokine balance and cross-talk among endosomal nucleic acid–sensing receptors, influencing antigen presentation and downstream adaptive immunity. Dysregulated TLR8-linked signaling has been associated with inflammatory phenotypes and altered host defense, making it relevant for studies of infection biology, autoimmunity mechanisms, and immunometabolic stress.
TLR8 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Tlr8 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Tlr8 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Tlr8 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TLR8 protein expression.
This CRISPR knockout system enables efficient generation of Tlr8-deficient cell models for investigation of TLR8 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Tlr8 exon(s) critical for TLR8 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Tlr8 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TLR8 CRISPR/Cas9 KO Plasmid (m) and TLR8 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Tlr8 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TLR8 HDR Plasmid (m) and TLR8 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Tlr8 homology arms to support homology-directed repair at defined Tlr8 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.