TLR5 Antibody (19D759.2) recommended for detection of TLR5 of mouse, rat and human origin by WB, IF, IHC(P) and FCM

TLR5 Antibody (19D759.2): sc-57461

TLR5 Antibody (19D759.2) is rated 4.0 out of 5 by 1.
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Datasheets

    • TLR5 Antibody (19D759.2) is a mouse monoclonal IgG2a (kappa light chain) provided at 100 µg/ml
    • raised against acids 700-800 of TLR5 of human origin
    • recommended for detection of TLR5 of mouse, rat and human origin by WB, IF, IHC(P) and FCM; also reactive with additional species, including canine
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of TLR5 (19D759.2): sc-57461.
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Can I use this antibody to stain TLR5 in mouse intestinal sections fixed with PFA?

Asked by: AnaR
Thank you for your question. This antibody sc-57461 is recommended for detection of TLR5 of mouse, rat and human origin by Western Blot, Immunocytochemistry on methanol-fixed cells, Immunohistochemistry on acetone-fixed frozen tissues sections, Immunohistochemistry on paraffin-embedded tissues sections fixed with formalin and FCM; This antibody is also reactive with additional species, including canine.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-07

Can TLR5 (19D759.2): sc-57461 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Cweed
Thank you for your question. Yes, TLR5 (19D759.2): sc-57461 is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-04-05
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Rated 4 out of 5 by from Flow Cytometry publication using human MDSC Flow Cytometry publication using human MDSC samples -SCBT Publication Review
Date published: 2015-03-28
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