TLR5 Antibody (19D759.2): sc-57461

TLR5 Antibody (19D759.2) is a mouse monoclonal IgG2a kappa light chain antibody that detects TLR5 in mouse, rat, and human samples through applications such as western blotting (WB), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). Anti-TLR5 antibody (19D759.2) is available in a non-conjugated form, allowing for versatile experimental use. TLR5, a member of the Toll-like receptor family, plays a crucial role in the innate immune response by recognizing bacterial flagellin, which is essential for the activation of immune signaling pathways. TLR5 is predominantly located on the cell surface of immune cells, particularly in monocytes and macrophages, where TLR5 initiates a cascade of immune responses upon detection of pathogens. TLR5 expression in these immune cells is vital for the early detection of infections, as TLR5 triggers the production of pro-inflammatory cytokines and enhances the overall immune response. TLR5 is highly expressed in the ovary and peripheral blood leukocytes, indicating TLR5′s importance in both reproductive and immune functions. Understanding TLR5′s role in immune signaling not only sheds light on host defense mechanisms but also provides insights into potential therapeutic targets for inflammatory diseases and infections.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

TLR5 Antibody (19D759.2) References:

1. Toll-like receptor-4 mediates lipopolysaccharide-induced signal transduction.  |  Chow, JC., et al. 1999. J Biol Chem. 274: 10689-92. PMID: 10196138
2. TLR6: A novel member of an expanding toll-like receptor family.  |  Takeuchi, O., et al. 1999. Gene. 231: 59-65. PMID: 10231569
3. Peptidoglycan- and lipoteichoic acid-induced cell activation is mediated by toll-like receptor 2.  |  Schwandner, R., et al. 1999. J Biol Chem. 274: 17406-9. PMID: 10364168
4. Host defense mechanisms triggered by microbial lipoproteins through toll-like receptors.  |  Brightbill, HD., et al. 1999. Science. 285: 732-6. PMID: 10426995
5. Drosophila Toll and IL-1 receptor.  |  Gay, NJ. and Keith, FJ. 1991. Nature. 351: 355-6. PMID: 1851964
6. The Effect of Denatured Flagellin on Toll-Like Receptor-5 (TLR-5) in Mice.  |  Mcheik, S., et al. 2018. Endocr Metab Immune Disord Drug Targets. 18: 412-416. PMID: 29589550
7. Roseburia intestinalis stimulates TLR5-dependent intestinal immunity against Crohn's disease.  |  Shen, Z., et al. 2022. EBioMedicine. 85: 104285. PMID: 36182776
8. A Clinical Trial of Entolimod a TLR-5 Adjuvant for Vaccines Using Diphtheria or Tetanus as Carrier Proteins.  |  Kosten, TR., et al. 2022. Vaccines (Basel). 10: PMID: 36298456
9. Mucosal TLR5 activation controls healthspan and longevity.  |  Lim, JS., et al. 2024. Nat Commun. 15: 46. PMID: 38167804
10. A human homologue of the Drosophila Toll protein signals activation of adaptive immunity.  |  Medzhitov, R., et al. 1997. Nature. 388: 394-7. PMID: 9237759
11. A family of human receptors structurally related to Drosophila Toll.  |  Rock, FL., et al. 1998. Proc Natl Acad Sci U S A. 95: 588-93. PMID: 9435236
12. Toll-like receptor-2 mediates lipopolysaccharide-induced cellular signalling.  |  Yang, RB., et al. 1998. Nature. 395: 284-8. PMID: 9751057

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TLR5 Antibody (19D759.2) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-TLR5 Antibody (19D759.2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TLR5 Antibody (19D759.2) Specifications

Alternate Product Name: TLR5

Product Clone Name: 19D759.2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 700-800

Epitope Target: TLR5

Epitope Species: human

Product Reactivity/Detection: TLR5

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IF, IHC(P), FCM

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for TLR5 Antibody (19D759.2): N/A

The preferred TLR5 antibody is TLR5 Antibody (H-11): sc-518106.