TLF Antibody (C-8): sc-514059
- TLF Antibody (C-8) is a mouse monoclonal IgG2a κ TLF antibody provided at 200 µg/ml
- raised against amino acids 77-186 mapping at the C-terminus of TLF of human origin
- TLF Antibody (C-8) is recommended for detection of TLF of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-TLF Antibody (C-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for TLF Antibody (C-8) for WB and IHC(P) applications. These reagents are now offered in bundles with TLF Antibody (C-8) (see ordering information below).
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TLF Antibody (C-8) is a mouse monoclonal IgG2a kappa light chain antibody that detects TLF protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). anti-TLF antibody (C-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TLF protein, also known as TBP-related factor 2 (TRF2), plays a crucial role in gene regulation by acting as a gene-specific factor for RNA polymerase II-mediated transcription. Unlike TATA box-binding protein (TBP), which is a universal binding factor for various RNA polymerase complexes, TLF selectively interacts with specific promoters, thereby influencing the expression of genes such as neurofibromatosis type 1 (NF1) gene. TLF′s ability to preferentially bind to and form stable complexes with TFIIA is particularly important, as TFIIA enhances TBP/TFIID binding to DNA and mitigates repression by negative cofactors, thus facilitating effective transcriptional activation. Unique structural features of TLF, which are related to those of TBP and Drosophila TBP-related factor TRF1, underscore TLF′s specialized function in transcriptional machinery, making TLF (C-8) antibody an essential tool for researchers studying gene expression and regulation in various biological contexts.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
TLF Antibody (C-8) References:
- TATA box-binding protein (TBP)-related factor 2 (TRF2), a third member of the TBP family. | Rabenstein, MD., et al. 1999. Proc Natl Acad Sci U S A. 96: 4791-6. PMID: 10220372
- Transcriptional functions of a new mammalian TATA-binding protein-related factor. | Maldonado, E. 1999. J Biol Chem. 274: 12963-6. PMID: 10224042
- Human TATA-binding protein-related factor-2 (hTRF2) stably associates with hTFIIA in HeLa cells. | Teichmann, M., et al. 1999. Proc Natl Acad Sci U S A. 96: 13720-5. PMID: 10570139
- A testis-specific transcription factor IIA (TFIIAtau) stimulates TATA-binding protein-DNA binding and transcription activation. | Ozer, J., et al. 2000. J Biol Chem. 275: 122-8. PMID: 10617594
- Family of proteins that interact with TFIID and regulate promoter activity. | Meisterernst, M. and Roeder, RG. 1991. Cell. 67: 557-67. PMID: 1934060
- The general transcription factors of RNA polymerase II. | Orphanides, G., et al. 1996. Genes Dev. 10: 2657-83. PMID: 8946909
- Regulation of gene expression by TBP-associated proteins. | Lee, TI. and Young, RA. 1998. Genes Dev. 12: 1398-408. PMID: 9585500
- Transcription factor IIA derepresses TATA-binding protein (TBP)-associated factor inhibition of TBP-DNA binding. | Ozer, J., et al. 1998. J Biol Chem. 273: 14293-300. PMID: 9603936
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TLF Antibody (C-8) | sc-514059 | 200 µg/ml | $322.00 | |||
TLF Antibody (C-8): m-IgG Fc BP-HRP Bundle | sc-531017 | 200 µg Ab; 10 µg BP | $361.00 | |||
TLF Antibody (C-8): m-IgGκ BP-HRP Bundle | sc-524591 | 200 µg Ab, 40 µg BP | $361.00 | |||
TLF Antibody (C-8): m-IgG2a BP-HRP Bundle | sc-547663 | 200 µg Ab; 10 µg BP | $361.00 | |||
TLF Antibody (C-8) AC | sc-514059 AC | 500 µg/ml, 25% agarose | $424.00 | |||
TLF Antibody (C-8) HRP | sc-514059 HRP | 200 µg/ml | $322.00 | |||
TLF Antibody (C-8) FITC | sc-514059 FITC | 200 µg/ml | $336.00 | |||
TLF Antibody (C-8) PE | sc-514059 PE | 200 µg/ml | $349.00 | |||
TLF Antibody (C-8) Alexa Fluor® 488 | sc-514059 AF488 | 200 µg/ml | $364.00 | |||
TLF Antibody (C-8) Alexa Fluor® 546 | sc-514059 AF546 | 200 µg/ml | $364.00 | |||
TLF Antibody (C-8) Alexa Fluor® 594 | sc-514059 AF594 | 200 µg/ml | $364.00 | |||
TLF Antibody (C-8) Alexa Fluor® 647 | sc-514059 AF647 | 200 µg/ml | $364.00 | |||
TLF Antibody (C-8) Alexa Fluor® 680 | sc-514059 AF680 | 200 µg/ml | $364.00 | |||
TLF Antibody (C-8) Alexa Fluor® 790 | sc-514059 AF790 | 200 µg/ml | $364.00 |