TLE6 Antibody (D-4): sc-515065
- TLE6 Antibody (D-4) is a mouse monoclonal IgG1 λ TLE6 antibody, cited in 1 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 58-77 near the N-terminus of TLE6 of mouse origin
- TLE6 Antibody (D-4) is recommended for detection of TLE6 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-TLE6 Antibody (D-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGλ BP-HRP (mouse IgGλ binding protein-HRP) is the preferred secondary detection reagent for TLE6 Antibody (D-4) for WB applications. This reagent is now offered in a bundle with TLE6 Antibody (D-4) (see ordering information below). For additional m-IgGλ BP conjugates see our complete list of Mouse IgG Binding Proteins.
TLE6 Antibody (D-4) is a mouse monoclonal IgG1 antibody that detects TLE6 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TLE6 (D-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TLE6 protein, also known as transducin-like enhancer of split 6 or GRG6, is a 449 amino acid cytoplasmic protein that plays a crucial role in the Notch signaling pathway, which regulates cellular interactions and fate specification in both vertebrates and invertebrates. As a member of the subcortical maternal complex (SCMC), TLE6 is vital for the progression of zygotes beyond the initial embryonic cell divisions, highlighting its importance in early development. Structurally, TLE6 contains seven WD repeats, a motif that facilitates protein-protein interactions, suggesting involvement in various cellular processes such as signal transduction, RNA processing, gene regulation, vesicular trafficking, and cytoskeletal assembly. TLE6′s ability to interact with other proteins within these pathways underscores significance in maintaining cellular functions and developmental processes, making anti-TLE6 antibody (D-4) an invaluable tool for researchers studying these critical biological mechanisms.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
TLE6 Antibody (D-4) References:
- Inhibition of nuclear factor-kappaB-mediated transcription by association with the amino-terminal enhancer of split, a Groucho-related protein lacking WD40 repeats. | Tetsuka, T., et al. 2000. J Biol Chem. 275: 4383-90. PMID: 10660609
- Transducin-like enhancer of split proteins, the human homologs of Drosophila groucho, interact with hepatic nuclear factor 3beta. | Wang, JC., et al. 2000. J Biol Chem. 275: 18418-23. PMID: 10748198
- Pf1, a novel PHD zinc finger protein that links the TLE corepressor to the mSin3A-histone deacetylase complex. | Yochum, GS. and Ayer, DE. 2001. Mol Cell Biol. 21: 4110-8. PMID: 11390640
- Antagonistic effects of Grg6 and Groucho/TLE on the transcription repression activity of brain factor 1/FoxG1 and cortical neuron differentiation. | Marçal, N., et al. 2005. Mol Cell Biol. 25: 10916-29. PMID: 16314515
- Evolution of the Groucho/Tle gene family: gene organization and duplication events. | Bajoghli, B. 2007. Dev Genes Evol. 217: 613-8. PMID: 17624551
- Repression by Groucho/TLE/Grg proteins: genomic site recruitment generates compacted chromatin in vitro and impairs activator binding in vivo. | Sekiya, T. and Zaret, KS. 2007. Mol Cell. 28: 291-303. PMID: 17964267
- Function of multiple Lis-Homology domain/WD-40 repeat-containing proteins in feed-forward transcriptional repression by silencing mediator for retinoic and thyroid receptor/nuclear receptor corepressor complexes. | Choi, HK., et al. 2008. Mol Endocrinol. 22: 1093-104. PMID: 18202150
- The Groucho/TLE/Grg family of transcriptional co-repressors. | Jennings, BH. and Ish-Horowicz, D. 2008. Genome Biol. 9: 205. PMID: 18254933
- Novel roles for MLH3 deficiency and TLE6-like amplification in DNA mismatch repair-deficient gastrointestinal tumorigenesis and progression. | Chen, PC., et al. 2008. PLoS Genet. 4: e1000092. PMID: 18551179
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TLE6 Antibody (D-4) | sc-515065 | 200 µg/ml | $322.00 | |||
TLE6 Antibody (D-4): m-IgGλ BP-HRP Bundle | sc-524924 | 200 µg Ab, 40 µg BP | $361.00 | |||
TLE6 Antibody (D-4) AC | sc-515065 AC | 500 µg/ml, 25% agarose | $424.00 | |||
TLE6 Antibody (D-4) HRP | sc-515065 HRP | 200 µg/ml | $322.00 | |||
TLE6 Antibody (D-4) FITC | sc-515065 FITC | 200 µg/ml | $336.00 | |||
TLE6 Antibody (D-4) PE | sc-515065 PE | 200 µg/ml | $349.00 | |||
TLE6 Antibody (D-4) Alexa Fluor® 488 | sc-515065 AF488 | 200 µg/ml | $364.00 | |||
TLE6 Antibody (D-4) Alexa Fluor® 546 | sc-515065 AF546 | 200 µg/ml | $364.00 | |||
TLE6 Antibody (D-4) Alexa Fluor® 594 | sc-515065 AF594 | 200 µg/ml | $364.00 | |||
TLE6 Antibody (D-4) Alexa Fluor® 647 | sc-515065 AF647 | 200 µg/ml | $364.00 | |||
TLE6 Antibody (D-4) Alexa Fluor® 680 | sc-515065 AF680 | 200 µg/ml | $364.00 | |||
TLE6 Antibody (D-4) Alexa Fluor® 790 | sc-515065 AF790 | 200 µg/ml | $364.00 | |||
TLE6 (D-4) Neutralizing Peptide | sc-515065 P | 100 µg/0.5 ml | $69.00 |