TLE5 Antibody (C-7): sc-515756
- TLE5 Antibody (C-7) is a mouse monoclonal IgG2b κ TLE5 antibody provided at 200 µg/ml
- raised against amino acids 127-197 mapping at the C-terminus of TLE5 of human origin
- TLE5 Antibody (C-7) is recommended for detection of TLE5 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-TLE5 Antibody (C-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGκ BP-HRP is the preferred secondary detection reagent for TLE5 Antibody (C-7) for WB applications. This reagent is now offered in a bundle with TLE5 Antibody (C-7) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
TLE5 Antibody (C-7) is a mouse monoclonal IgG2b kappa light chain antibody that detects TLE5 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TLE5 (C-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TLE5, also known as AES (amino-terminal enhancer of split), GRG, or ESP1, is a 197 amino acid nuclear protein that plays a crucial role in the Notch signaling pathway, which is essential for regulating cellular interactions that determine cell fate in both vertebrates and invertebrates. TLE5 functions primarily as a transcriptional repressor, either as a homooligomer or in complex with other TLE family members, thereby inhibiting the expression of target genes involved in cell differentiation. TLE5 can also repress NFκB-regulated gene expression, highlighting its importance in maintaining cellular homeostasis and differentiation processes. TLE5 expression is predominantly observed in fetal brain, liver, lung, heart, and kidney tissues, as well as in adult muscle, indicating its significant role during development and in adult tissue function. Two isoforms of TLE5 arise from alternative splicing, which may contribute to its functional diversity and regulatory mechanisms in various biological contexts.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
TLE5 Antibody (C-7) References:
- Inhibition of nuclear factor-kappaB-mediated transcription by association with the amino-terminal enhancer of split, a Groucho-related protein lacking WD40 repeats. | Tetsuka, T., et al. 2000. J Biol Chem. 275: 4383-90. PMID: 10660609
- Transducin-like enhancer of split proteins, the human homologs of Drosophila groucho, interact with hepatic nuclear factor 3beta. | Wang, JC., et al. 2000. J Biol Chem. 275: 18418-23. PMID: 10748198
- Pf1, a novel PHD zinc finger protein that links the TLE corepressor to the mSin3A-histone deacetylase complex. | Yochum, GS. and Ayer, DE. 2001. Mol Cell Biol. 21: 4110-8. PMID: 11390640
- Six3 and Six6 activity is modulated by members of the groucho family. | López-Ríos, J., et al. 2003. Development. 130: 185-95. PMID: 12441302
- A mitochondrial protein, Bit1, mediates apoptosis regulated by integrins and Groucho/TLE corepressors. | Jan, Y., et al. 2004. Cell. 116: 751-62. PMID: 15006356
- Repression by Groucho/TLE/Grg proteins: genomic site recruitment generates compacted chromatin in vitro and impairs activator binding in vivo. | Sekiya, T. and Zaret, KS. 2007. Mol Cell. 28: 291-303. PMID: 17964267
- The Groucho/TLE/Grg family of transcriptional co-repressors. | Jennings, BH. and Ish-Horowicz, D. 2008. Genome Biol. 9: 205. PMID: 18254933
- Molecular cloning and expression of mouse and human cDNA encoding AES and ESG proteins with strong similarity to Drosophila enhancer of split groucho protein. | Miyasaka, H., et al. 1993. Eur J Biochem. 216: 343-52. PMID: 8365415
- Genomic organization and chromosome localization to band 19p13.3 of the human AES gene: gene product exhibits strong similarity to the N-terminal domain of Drosophila enhancer of Split Groucho protein. | Hou, EW. and Li, SS. 1998. DNA Cell Biol. 17: 911-3. PMID: 9809752
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TLE5 Antibody (C-7) | sc-515756 | 200 µg/ml | $322.00 | |||
TLE5 Antibody (C-7): m-IgGκ BP-HRP Bundle | sc-525200 | 200 µg Ab, 40 µg BP | $361.00 | |||
TLE5 Antibody (C-7) AC | sc-515756 AC | 500 µg/ml, 25% agarose | $424.00 | |||
TLE5 Antibody (C-7) HRP | sc-515756 HRP | 200 µg/ml | $322.00 | |||
TLE5 Antibody (C-7) FITC | sc-515756 FITC | 200 µg/ml | $336.00 | |||
TLE5 Antibody (C-7) PE | sc-515756 PE | 200 µg/ml | $349.00 | |||
TLE5 Antibody (C-7) Alexa Fluor® 488 | sc-515756 AF488 | 200 µg/ml | $364.00 | |||
TLE5 Antibody (C-7) Alexa Fluor® 546 | sc-515756 AF546 | 200 µg/ml | $364.00 | |||
TLE5 Antibody (C-7) Alexa Fluor® 594 | sc-515756 AF594 | 200 µg/ml | $364.00 | |||
TLE5 Antibody (C-7) Alexa Fluor® 647 | sc-515756 AF647 | 200 µg/ml | $364.00 | |||
TLE5 Antibody (C-7) Alexa Fluor® 680 | sc-515756 AF680 | 200 µg/ml | $364.00 | |||
TLE5 Antibody (C-7) Alexa Fluor® 790 | sc-515756 AF790 | 200 µg/ml | $364.00 |