Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
TLE2 CRISPR/Cas9 KO Plasmid (m): sc-423413
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TLE2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TLE2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TLE2 Antibody (D-10): sc-374226
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TLE2 CRISPR/Cas9 KO Plasmid (m) | sc-423413 | 20 µg | $397.00 |
Overview
Tle2 encodes the transcriptional corepressor TLE2, a Groucho/TLE family protein that scaffolds repressive chromatin complexes to modulate context-dependent gene expression programs during development and tissue homeostasis. TLE2 interacts with sequence-specific transcription factors and integrates signals from pathways including Wnt/β-catenin and Notch, shaping cell fate decisions, differentiation, and stem/progenitor maintenance. In mouse models and cellular systems, altered TLE2 activity can perturb lineage specification and proliferative control, making it relevant for studying mechanisms underlying dysregulated transcriptional networks in developmental disorders and cancer-associated pathways. Its nuclear corepressor functions also position it as a useful node for dissecting epigenetic regulation and transcriptional repression dynamics.
TLE2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Tle2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Tle2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Tle2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TLE2 protein expression.
This CRISPR knockout system enables efficient generation of Tle2-deficient cell models for investigation of TLE2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Tle2 exon(s) critical for TLE2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Tle2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TLE2 CRISPR/Cas9 KO Plasmid (m) and TLE2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Tle2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TLE2 HDR Plasmid (m) and TLE2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Tle2 homology arms to support homology-directed repair at defined Tle2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.