Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
TLE1 CRISPR/Cas9 KO Plasmid (m): sc-423412
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TLE1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TLE1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TLE1 Antibody (F-4): sc-137098
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TLE1 CRISPR/Cas9 KO Plasmid (m) | sc-423412 | 20 µg | $397.00 |
Overview
Tle1 encodes the transcriptional corepressor TLE1 (Transducin-Like Enhancer of split 1), a Groucho/TLE family member that modulates gene expression by binding DNA-associated transcription factors and recruiting chromatin-regulatory complexes. In mouse cells, TLE1 is a key effector of Notch and Wnt/β-catenin-responsive transcriptional programs and contributes to lineage specification, neuronal development, and maintenance of cellular identity through repression of target promoters and enhancers. By integrating signals from developmental pathways, TLE1 influences cell-fate decisions, differentiation timing, and context-dependent proliferation. Altered TLE1-linked transcriptional control is studied in models of developmental dysregulation and oncogenic signaling where Wnt/Notch pathway balance and epigenetic repression are perturbed.
TLE1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Tle1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Tle1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Tle1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TLE1 protein expression.
This CRISPR knockout system enables efficient generation of Tle1-deficient cell models for investigation of TLE1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Tle1 exon(s) critical for TLE1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Tle1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TLE1 CRISPR/Cas9 KO Plasmid (m) and TLE1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Tle1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TLE1 HDR Plasmid (m) and TLE1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Tle1 homology arms to support homology-directed repair at defined Tle1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.