TLE1 Antibody (F-4): sc-137098

TLE1 Antibody (F-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects TLE1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TLE1 (F-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TLE1, also known as Enhancer of Split Groucho-like protein 1 (ESG1), plays a crucial role in the Notch signaling pathway, which is essential for regulating cellular interactions that determine cell fate during development in both invertebrates and vertebrates. TLE1 functions as a transcriptional repressor, interacting with chromatin and specifically binding to Histone H3, thereby influencing gene expression during neurogenesis, segmentation, and sex determination. TLE1 is involved in the differentiation of epithelial cells, with expression correlating with immature epithelial cells transitioning toward a terminally differentiated state. Elevated levels of TLE1, along with TLE2 and TLE3, have been observed in cervical squamous metaplasias and carcinomas, highlighting a potential role in cancer biology. TLE1 contains SP and WD40 domains, which facilitate interaction with other proteins, such as AML1, to inhibit transactivation of the CSF1 receptor. Dynamic expression patterns of TLE1, TLE2, and TLE4 during various stages of cell differentiation underscore the complexity of regulatory roles in cellular processes.

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TLE1 Antibody (F-4) References:

1. Human homologs of a Drosophila Enhancer of split gene product define a novel family of nuclear proteins.  |  Stifani, S., et al. 1992. Nat Genet. 2: 119-27. PMID: 1303260
2. Molecular recognition of transcriptional repressor motifs by the WD domain of the Groucho/TLE corepressor.  |  Jennings, BH., et al. 2006. Mol Cell. 22: 645-55. PMID: 16762837
3. TLE1 as a diagnostic immunohistochemical marker for synovial sarcoma emerging from gene expression profiling studies.  |  Terry, J., et al. 2007. Am J Surg Pathol. 31: 240-6. PMID: 17255769
4. Aristaless-related homeobox gene, the gene responsible for West syndrome and related disorders, is a Groucho/transducin-like enhancer of split dependent transcriptional repressor.  |  McKenzie, O., et al. 2007. Neuroscience. 146: 236-47. PMID: 17331656
5. Ras-Erk signaling induces phosphorylation of human TLE1 and downregulates its repressor function.  |  Zahavi, T., et al. 2017. Oncogene. 36: 3729-3739. PMID: 28192406
6. TLE1 inhibits anoikis and promotes tumorigenicity in human lung cancer cells through ZEB1-mediated E-cadherin repression.  |  Yao, X., et al. 2017. Oncotarget. 8: 72235-72249. PMID: 29069783
7. Foxq1 Promotes Alveolar Epithelial Cell Death through Tle1-mediated Inhibition of the NF-κB Signaling Pathway.  |  Zhu, X., et al. 2024. Am J Respir Cell Mol Biol. 71: 53-65. PMID: 38574238
8. Groucho is required for Drosophila neurogenesis, segmentation, and sex determination and interacts directly with hairy-related bHLH proteins.  |  Paroush, Z., et al. 1994. Cell. 79: 805-15. PMID: 8001118
9. Epithelial expression and chromosomal location of human TLE genes: implications for notch signaling and neoplasia.  |  Liu, Y., et al. 1996. Genomics. 31: 58-64. PMID: 8808280
10. The Groucho/transducin-like enhancer of split transcriptional repressors interact with the genetically defined amino-terminal silencing domain of histone H3.  |  Palaparti, A., et al. 1997. J Biol Chem. 272: 26604-10. PMID: 9334241
11. Combinatorial expression patterns of individual TLE proteins during cell determination and differentiation suggest non-redundant functions for mammalian homologs of Drosophila Groucho.  |  Yao, J., et al. 1998. Dev Growth Differ. 40: 133-46. PMID: 9572356
12. Transcriptional repression by AML1 and LEF-1 is mediated by the TLE/Groucho corepressors.  |  Levanon, D., et al. 1998. Proc Natl Acad Sci U S A. 95: 11590-5. PMID: 9751710

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TLE1 Antibody (F-4) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-TLE1 Antibody (F-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TLE1 Antibody (F-4) Specifications

Alternate Product Name: TLE1

Product Clone Name: F-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 200-350

Epitope Target: TLE1

Epitope Species: mouse

Product Reactivity/Detection: TLE1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for TLE1 Antibody (F-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent

The preferred TLE4 antibody is TLE4 Antibody (E-10): sc-365406.