TIRAP Antibody (A-11): sc-166149

TIRAP Antibody (A-11) is a mouse monoclonal IgG2a kappa light chain antibody that detects TIRAP protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TIRAP Antibody (A-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TIRAP protein, also known as MyD88 adapter-like protein, is crucial for the innate immune response as anti-TIRAP antibody (A-11) serves as an adapter in the signaling pathways activated by Toll-like receptors (TLRs). Specifically, TIRAP is located in the cytoplasm and plays a pivotal role in the transduction of signals from TLR-4, which is essential for the activation of downstream signaling cascades, including NFκB and mitogen-activated protein kinases. This localization allows TIRAP to rapidly respond to pathogen-associated molecular patterns, facilitating a swift immune response. TIRAP′s ability to form homodimers and heterodimers with MyD88 enhances functionality in the immune signaling pathway, making TIRAP a critical component in the body′s defense against infections.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

TIRAP Antibody (A-11) References:

1. Toll-like receptor-4 mediates lipopolysaccharide-induced signal transduction.  |  Chow, JC., et al. 1999. J Biol Chem. 274: 10689-92. PMID: 10196138
2. The biology of Toll-like receptors.  |  Means, TK., et al. 2000. Cytokine Growth Factor Rev. 11: 219-32. PMID: 10817965
3. TIRAP: an adapter molecule in the Toll signaling pathway.  |  Horng, T., et al. 2001. Nat Immunol. 2: 835-41. PMID: 11526399
4. Mal (MyD88-adapter-like) is required for Toll-like receptor-4 signal transduction.  |  Fitzgerald, KA., et al. 2001. Nature. 413: 78-83. PMID: 11544529
5. Association of TLR1, TLR2, TLR4, TLR6, and TIRAP polymorphisms with disease susceptibility.  |  Noreen, M. and Arshad, M. 2015. Immunol Res. 62: 234-52. PMID: 25784622
6. Paradoxical Roles of the MAL/Tirap Adaptor in Pathologies.  |  Belhaouane, I., et al. 2020. Front Immunol. 11: 569127. PMID: 33072109
7. TIRAP in the Mechanism of Inflammation.  |  Rajpoot, S., et al. 2021. Front Immunol. 12: 697588. PMID: 34305934
8. TIRAP drives myelosuppression through an Ifnγ-Hmgb1 axis that disrupts the endothelial niche in mice.  |  Gopal, A., et al. 2022. J Exp Med. 219: PMID: 35089323
9. TIRAP-mediated activation of p38 MAPK in inflammatory signaling.  |  Rajpoot, S., et al. 2022. Sci Rep. 12: 5601. PMID: 35379857
10. Tirap controls Mycobacterium tuberculosis phagosomal acidification.  |  Belhaouane, I., et al. 2023. PLoS Pathog. 19: e1011192. PMID: 36888688
11. MyD88, an adapter protein involved in interleukin-1 signaling.  |  Burns, K., et al. 1998. J Biol Chem. 273: 12203-9. PMID: 9575168
12. MyD88 is an adaptor protein in the hToll/IL-1 receptor family signaling pathways.  |  Medzhitov, R., et al. 1998. Mol Cell. 2: 253-8. PMID: 9734363

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TIRAP Antibody (A-11) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-TIRAP Antibody (A-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TIRAP Antibody (A-11) Specifications

Alternate Product Name: TIRAP

Product Clone Name: A-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-235

Epitope Target: TIRAP

Epitope Species: human

Product Reactivity/Detection: TIRAP

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for TIRAP Antibody (A-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin