TIP30 Antibody (F-10): sc-515728
- TIP30 Antibody (F-10) is a mouse monoclonal IgG1 κ TIP30 antibody provided at 200 µg/ml
- raised against amino acids 145-217 mapping near the C-terminus of TIP30 of human origin
- TIP30 Antibody (F-10) is recommended for detection of TIP30 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-TIP30 Antibody (F-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for TIP30 Antibody (F-10). This work is in progress.
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TIP30 Antibody (F-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects TIP30 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TIP30 (F-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TIP30 protein, also known as HTATIP2 or CC3, is a 30 kDa HIV-1 TAT-interacting protein that plays a crucial role as an oxidoreductase and tumor suppressor. TIP30 function is vital in metabolic suppression, inhibition of angiogenesis, and induction of apoptosis, making TIP30 a significant player in cancer biology. TIP30 is predominantly located in the cytoplasm and nucleus, where TIP30 interacts with various proteins, including the estrogen receptor alpha (ERα) coactivator NCoA-5, thereby regulating c-Myc transcription in response to estrogen signaling. When TIP30 is overexpressed, TIP30 represses c-Myc transcription mediated by ERα, while TIP30 deficiency leads to increased c-Myc transcription, highlighting TIP30′s importance in maintaining cellular homeostasis. Additionally, TIP30 enhances the transcriptional activity of HIV-1 TAT by phosphorylating RNA polymerase II, linking TIP30 to viral pathogenesis. Defects in TIP30 have been associated with hepatocellular carcinomas and apoptotic-resistant tumor cells, suggesting TIP30′s potential as a therapeutic target in cancer treatment.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
TIP30 Antibody (F-10) References:
- TIP30 deficiency increases susceptibility to tumorigenesis. | Ito, M., et al. 2003. Cancer Res. 63: 8763-7. PMID: 14695192
- TIP30 interacts with an estrogen receptor alpha-interacting coactivator CIA and regulates c-myc transcription. | Jiang, C., et al. 2004. J Biol Chem. 279: 27781-9. PMID: 15073177
- TIP30 regulates apoptosis-related genes in its apoptotic signal transduction pathway. | Shi, M., et al. 2005. World J Gastroenterol. 11: 221-7. PMID: 15633220
- Crystal structure of CC3 (TIP30): implications for its role as a tumor suppressor. | El Omari, K., et al. 2005. J Biol Chem. 280: 18229-36. PMID: 15728189
- Overexpression of CC3/TIP30 is associated with HER-2/neu status in breast cancer. | Zhang, DH., et al. 2005. J Cancer Res Clin Oncol. 131: 603-8. PMID: 15931508
- TIP30/CC3 expression in breast carcinoma: relation to metastasis, clinicopathologic parameters, and P53 expression. | Zhao, J., et al. 2007. Hum Pathol. 38: 293-8. PMID: 17097132
- TIP30 mutant derived from hepatocellular carcinoma specimens promotes growth of HepG2 cells through up-regulation of N-cadherin. | Jiang, C., et al. 2007. Cancer Res. 67: 3574-82. PMID: 17440068
- Deletion of Tip30 leads to rapid immortalization of murine mammary epithelial cells and ductal hyperplasia in the mammary gland. | Pecha, J., et al. 2007. Oncogene. 26: 7423-31. PMID: 17533366
- Tip30-induced apoptosis requires translocation of Bax and involves mitochondrial release of cytochrome c and Smac/DIABLO in hepatocellular carcinoma cells. | Shi, M., et al. 2008. Biochim Biophys Acta. 1783: 263-74. PMID: 17997990
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TIP30 Antibody (F-10) | sc-515728 | 200 µg/ml | $322.00 | |||
TIP30 Antibody (F-10) AC | sc-515728 AC | 500 µg/ml, 25% agarose | $424.00 | |||
TIP30 Antibody (F-10) HRP | sc-515728 HRP | 200 µg/ml | $322.00 | |||
TIP30 Antibody (F-10) FITC | sc-515728 FITC | 200 µg/ml | $336.00 | |||
TIP30 Antibody (F-10) PE | sc-515728 PE | 200 µg/ml | $349.00 | |||
TIP30 Antibody (F-10) Alexa Fluor® 488 | sc-515728 AF488 | 200 µg/ml | $364.00 | |||
TIP30 Antibody (F-10) Alexa Fluor® 546 | sc-515728 AF546 | 200 µg/ml | $364.00 | |||
TIP30 Antibody (F-10) Alexa Fluor® 594 | sc-515728 AF594 | 200 µg/ml | $364.00 | |||
TIP30 Antibody (F-10) Alexa Fluor® 647 | sc-515728 AF647 | 200 µg/ml | $364.00 | |||
TIP30 Antibody (F-10) Alexa Fluor® 680 | sc-515728 AF680 | 200 µg/ml | $364.00 | |||
TIP30 Antibody (F-10) Alexa Fluor® 790 | sc-515728 AF790 | 200 µg/ml | $364.00 |