TIM Antibody (H-11): sc-166785

TIM Antibody (H-11) is a mouse monoclonal IgG2a kappa light chain antibody that detects TIM protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). TIM (H-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Triosephosphate isomerase (TIM), also known as TPI1, plays a crucial role in the glycolytic pathway by catalyzing the reversible conversion of dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate, which are essential intermediates in glucose metabolism. This enzymatic activity is vital for energy production, as TIM facilitates the generation of pyruvate and adenosine triphosphate (ATP) from glucose, thereby supporting cellular respiration and energy homeostasis. The human TIM gene spans 3.5 kilobase pairs, consists of seven exons, and encodes a 249 amino acid protein. TIM expression regulation involves a promoter region containing a TATA box and multiple GC boxes, which are critical for transcription initiation. TIM deficiencies can lead to a rare autosomal recessive disorder characterized by a metabolic block in glycolysis, resulting in DHAP accumulation in erythrocytes and leading to serious health issues such as non-spherocytic hemolytic anemia, recurrent infections, cardiomyopathy, and neuromuscular dysfunctions.

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TIM Antibody (H-11) References:

1. Structural studies show that the A178L mutation in the C-terminal hinge of the catalytic loop-6 of triosephosphate isomerase (TIM) induces a closed-like conformation in dimeric and monomeric TIM.  |  Alahuhta, M., et al. 2008. Acta Crystallogr D Biol Crystallogr. 64: 178-88. PMID: 18219118
2. Transcriptional regulatory sequences of the housekeeping gene for human triosephosphate isomerase.  |  Boyer, TG., et al. 1989. J Biol Chem. 264: 5177-87. PMID: 2925688
3. A novel graphene oxide/trans-1,4-polyisoprene (GO/TPI) shape memory polymer nanocomposite and its multifunctional properties.  |  Liu, J., et al. 2019. Nanotechnology. 30: 255706. PMID: 30780136
4. Development of novel TPI/HDPE/CNTs ternary hybrid shape memory nanocomposites.  |  Wang, Z., et al. 2021. Nanotechnology. 32: PMID: 34192678
5. Tooth hop variability in human and nonhuman bone: Effect on the estimation of saw blade TPI.  |  Grosso, AR. 2022. J Forensic Sci. 67: 102-111. PMID: 34585386
6. Itavastatin and resveratrol increase triosephosphate isomerase protein in a newly identified variant of TPI deficiency.  |  VanDemark, AP., et al. 2022. Dis Model Mech. 15: PMID: 35315486
7. Expression of vimentin, TPI and MAT2A in human dermal microvascular endothelial cells during angiogenesis in vitro.  |  Herre, C., et al. 2022. PLoS One. 17: e0266774. PMID: 35482724
8. MIX-TPI: a flexible prediction framework for TCR-pMHC interactions based on multimodal representations.  |  Yang, M., et al. 2023. Bioinformatics. 39: PMID: 37527015
9. Knockdown of TPI in human dermal microvascular endothelial cells and its impact on angiogenesis in vitro.  |  Herre, C., et al. 2023. PLoS One. 18: e0294933. PMID: 38117832
10. A gene-rich cluster between the CD4 and triosephosphate isomerase genes at human chromosome 12p13.  |  Ansari-Lari, MA., et al. 1996. Genome Res. 6: 314-26. PMID: 8723724
11. Large-scale sequencing in human chromosome 12p13: experimental and computational gene structure determination.  |  Ansari-Lari, MA., et al. 1997. Genome Res. 7: 268-80. PMID: 9074930
12. The feasibility of replacement therapy for inherited disorder of glycolysis: triosephosphate isomerase deficiency (review).  |  Ationu, A. and Humphries, A. 1998. Int J Mol Med. 2: 701-4. PMID: 9850739

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TIM Antibody (H-11) has 13 citations in a variety of scientific publications.

Primary antibodies like Anti-TIM Antibody (H-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TIM Antibody (H-11) Specifications

Alternate Product Name: TIM

Product Clone Name: H-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-249

Epitope Target: TIM

Epitope Species: human

Product Reactivity/Detection: TIM

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 13 citations in various scientific publications

Available Conjugates/Forms for TIM Antibody (H-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin