TIF1α Antibody (C-4): sc-271266

TIF1α Antibody (C-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects TIF1 alpha protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-TIF1α antibody (C-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TIF1α plays a crucial role in mediating transcriptional regulation by interacting with the activation function 2 (AF2) region of several nuclear receptors, such as estrogen, retinoic acid, and vitamin D3 receptors, which are essential for various physiological processes including development, metabolism, and cellular differentiation. TIF1α is localized to nuclear bodies and associates with chromatin and heterochromatin-associated factors, highlighting its importance in gene expression regulation. As a member of the tripartite motif (TRIM) family, TIF1α contains three zinc-binding domains (RING, B-box type 1, and B-box type 2) along with a coiled-coil region, which are critical for structural integrity and functional interactions. TIF1α gene, located on human chromosome 7q34, encodes two alternatively spliced transcripts, although the full-length nature of one variant remains undetermined. A homolog of TIF1α, known as bonus, has been identified in Drosophila and is associated with genes implicated in the ecdysone pathway, a nuclear hormone receptor pathway vital for Drosophila development, suggesting a conserved functional role throughout evolution.

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TIF1α Antibody (C-4) References:

1. The transcription coactivator HTIF1 and a related protein are fused to the RET receptor tyrosine kinase in childhood papillary thyroid carcinomas.  |  Klugbauer, S. and Rabes, HM. 1999. Oncogene. 18: 4388-93. PMID: 10439047
2. Interaction with members of the heterochromatin protein 1 (HP1) family and histone deacetylation are differentially involved in transcriptional silencing by members of the TIF1 family.  |  Nielsen, AL., et al. 1999. EMBO J. 18: 6385-95. PMID: 10562550
3. Bonus, a Drosophila homolog of TIF1 proteins, interacts with nuclear receptors and can inhibit betaFTZ-F1-dependent transcription.  |  Beckstead, R., et al. 2001. Mol Cell. 7: 753-65. PMID: 11336699
4. Role of TIF1alpha as a modulator of embryonic transcription in the mouse zygote.  |  Torres-Padilla, ME. and Zernicka-Goetz, M. 2006. J Cell Biol. 174: 329-38. PMID: 16880268
5. Loss of Trim24 (Tif1alpha) gene function confers oncogenic activity to retinoic acid receptor alpha.  |  Khetchoumian, K., et al. 2007. Nat Genet. 39: 1500-6. PMID: 18026104
6. Arterial calcifications and increased expression of vitamin D receptor targets in mice lacking TIF1alpha.  |  Ignat, M., et al. 2008. Proc Natl Acad Sci U S A. 105: 2598-603. PMID: 18287084
7. Tripartite motif 24 (Trim24/Tif1α) tumor suppressor protein is a novel negative regulator of interferon (IFN)/signal transducers and activators of transcription (STAT) signaling pathway acting through retinoic acid receptor α (Rarα) inhibition.  |  Tisserand, J., et al. 2011. J Biol Chem. 286: 33369-79. PMID: 21768647
8. Adenovirus E4-ORF3-dependent relocalization of TIF1α and TIF1γ relies on access to the Coiled-Coil motif.  |  Vink, EI., et al. 2012. Virology. 422: 317-25. PMID: 22123502
9. The TIF1α-related TRIM cofactors couple chromatin modifications to transcriptional regulation, signaling and tumor suppression.  |  Herquel, B., et al. 2011. Transcription. 2: 231-6. PMID: 22231120
10. T-cell-intrinsic Tif1α/Trim24 regulates IL-1R expression on TH2 cells and TH2 cell-mediated airway allergy.  |  Perez-Lloret, J., et al. 2016. Proc Natl Acad Sci U S A. 113: E568-76. PMID: 26787865
11. The putative cofactor TIF1alpha is a protein kinase that is hyperphosphorylated upon interaction with liganded nuclear receptors.  |  Fraser, RA., et al. 1998. J Biol Chem. 273: 16199-204. PMID: 9632676
12. TIF1alpha: a possible link between KRAB zinc finger proteins and nuclear receptors.  |  Le Douarin, B., et al. 1998. J Steroid Biochem Mol Biol. 65: 43-50. PMID: 9699856

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TIF1α Antibody (C-4) has 13 citations in a variety of scientific publications.

Primary antibodies like Anti-TIF1α Antibody (C-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TIF1α Antibody (C-4) Specifications

Alternate Product Name: TIF1α

Product Clone Name: C-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 631-820

Epitope Target: TIF1α

Epitope Species: human

Product Reactivity/Detection: TIF1α long and short isoforms

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 13 citations in various scientific publications

Available Conjugates/Forms for TIF1α Antibody (C-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent