Tiam1 Antibody (E-7): sc-393315

Tiam1 Antibody (E-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects Tiam1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Tiam1 (E-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Tiam1 plays a crucial role in cellular signaling pathways, particularly in regulating the Rho family of GTPases, which are essential for cytoskeletal dynamics and cell motility. This function is vital for processes such as cell migration, invasion, and metastasis, making Tiam1 a significant player in cancer biology. Tiam1 contains both Dbl and Pleckstrin-homologous domains, which are characteristic of guanine nucleotide exchange factors that activate Rho-like proteins. In fibroblasts, Tiam1 can induce a phenotype similar to constitutively activated Rac1, leading to membrane ruffling, a process inhibited by dominant negative Rac1. T lymphoma cells expressing activated Rac1 exhibit increased invasiveness, highlighting the importance of Tiam1-Rac signaling in tumor cell invasion and metastasis.

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Tiam1 Antibody (E-7) References:

1. Ras-related GTPases and the cytoskeleton.  |  Hall, A. 1992. Mol Biol Cell. 3: 475-9. PMID: 1611153
2. Catalysis of guanine nucleotide exchange on the CDC42Hs protein by the dbl oncogene product.  |  Hart, MJ., et al. 1991. Nature. 354: 311-4. PMID: 1956381
3. Tiam1 is associated with hepatocellular carcinoma metastasis.  |  Huang, J., et al. 2013. Int J Cancer. 132: 90-100. PMID: 22573407
4. Balanced Tiam1-rac1 and RhoA drives proliferation and invasion of pancreatic cancer cells.  |  Guo, X., et al. 2013. Mol Cancer Res. 11: 230-9. PMID: 23322732
5. Clinicopathological implications of Tiam1 overexpression in invasive ductal carcinoma of the breast.  |  Li, Z., et al. 2016. BMC Cancer. 16: 681. PMID: 27562113
6. Decreased Tiam1-mediated Rac1 activation is responsible for impaired directional persistence of chondrocyte migration in microtia.  |  Wu, Y., et al. 2024. J Cell Mol Med. 28: e18443. PMID: 38837873
7. Inhibition of PMA-induced, LFA-1-dependent lymphocyte aggregation by ADP ribosylation of the small molecular weight GTP binding protein, rho.  |  Tominaga, T., et al. 1993. J Cell Biol. 120: 1529-37. PMID: 7680658
8. A role for Rac in Tiam1-induced membrane ruffling and invasion.  |  Michiels, F., et al. 1995. Nature. 375: 338-40. PMID: 7753201
9. A novel oncogene, ost, encodes a guanine nucleotide exchange factor that potentially links Rho and Rac signaling pathways.  |  Horii, Y., et al. 1994. EMBO J. 13: 4776-86. PMID: 7957046
10. Identification of an invasion-inducing gene, Tiam-1, that encodes a protein with homology to GDP-GTP exchangers for Rho-like proteins.  |  Habets, GG., et al. 1994. Cell. 77: 537-49. PMID: 7999144
11. Control of the yeast bud-site assembly GTPase Cdc42. Catalysis of guanine nucleotide exchange by Cdc24 and stimulation of GTPase activity by Bem3.  |  Zheng, Y., et al. 1994. J Biol Chem. 269: 2369-72. PMID: 8300560
12. Involvement of rho p21 and its inhibitory GDP/GTP exchange protein (rho GDI) in cell motility.  |  Takaishi, K., et al. 1993. Mol Cell Biol. 13: 72-9. PMID: 8417362

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Tiam1 Antibody (E-7) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-Tiam1 Antibody (E-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Tiam1 Antibody (E-7) Specifications

Alternate Product Name: Tiam1

Product Clone Name: E-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-300

Epitope Target: Tiam1

Epitope Species: human

Product Reactivity/Detection: Tiam1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for Tiam1 Antibody (E-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin