TI-VAMP Antibody (E-12): sc-166394
- TI-VAMP Antibody (E-12) is a mouse monoclonal IgG2b κ TI-VAMP antibody, cited in 3 publications, provided at 200 µg/ml
- raised against amino acids 91-145 mapping within an internal region of TI-VAMP of human origin
- TI-VAMP Antibody (E-12) is recommended for detection of TI-VAMP of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-TI-VAMP Antibody (E-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for TI-VAMP Antibody (E-12) for WB applications. These reagents are now offered in bundles with TI-VAMP Antibody (E-12) (see ordering information below).
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TI-VAMP Antibody (E-12) is a mouse monoclonal IgG2b kappa light chain antibody that detects TI-VAMP of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TI-VAMP (E-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TI-VAMP, also known as tetanus insensitive VAMP, is a type IV membrane protein that plays a crucial role in the exocytosis process, which is essential for neurotransmitter release and other cellular secretions. Located at the apical plasma membrane, TI-VAMP forms a SNARE complex with cellubrevin, facilitating the fusion of vesicles with target membranes. This interaction is vital for maintaining proper cellular communication and function, as TI-VAMP ensures that vesicles containing important signaling molecules are accurately delivered to their intended locations. Notably, TI-VAMP is resistant to clostridial neurotoxins, which distinguishes TI-VAMP from other VAMPs and highlights TI-VAMP′s significance in synaptic transmission and cellular signaling pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
TI-VAMP Antibody (E-12) References:
- VAMP-7 mediates vesicular transport from endosomes to lysosomes. | Advani, RJ., et al. 1999. J Cell Biol. 146: 765-76. PMID: 10459012
- Human and mouse SYBL1 gene structure and expression. | Matarazzo, MR., et al. 1999. Gene. 240: 233-8. PMID: 10564831
- Syntaxin 7 and VAMP-7 are soluble N-ethylmaleimide-sensitive factor attachment protein receptors required for late endosome-lysosome and homotypic lysosome fusion in alveolar macrophages. | Ward, DM., et al. 2000. Mol Biol Cell. 11: 2327-33. PMID: 10888671
- A SNARE complex mediating fusion of late endosomes defines conserved properties of SNARE structure and function. | Antonin, W., et al. 2000. EMBO J. 19: 6453-64. PMID: 11101518
- Association between a polymorphism in the pseudoautosomal X-linked gene SYBL1 and bipolar affective disorder. | Müller, DJ., et al. 2002. Am J Med Genet. 114: 74-8. PMID: 11840509
- Allelic inactivation of the pseudoautosomal gene SYBL1 is controlled by epigenetic mechanisms common to the X and Y chromosomes. | Matarazzo, MR., et al. 2002. Hum Mol Genet. 11: 3191-8. PMID: 12444103
- TI-VAMP/VAMP7 is required for optimal phagocytosis of opsonised particles in macrophages. | Braun, V., et al. 2004. EMBO J. 23: 4166-76. PMID: 15470500
- Multiple roles of the vesicular-SNARE TI-VAMP in post-Golgi and endosomal trafficking. | Chaineau, M., et al. 2009. FEBS Lett. 583: 3817-26. PMID: 19837067
- TI-VAMP/VAMP7 is the SNARE of secretory lysosomes contributing to ATP secretion from astrocytes. | Verderio, C., et al. 2012. Biol Cell. 104: 213-28. PMID: 22188132
- Absence of TI-VAMP/Vamp7 leads to increased anxiety in mice. | Danglot, L., et al. 2012. J Neurosci. 32: 1962-8. PMID: 22323709
- A synaptobrevin-like gene in the Xq28 pseudoautosomal region undergoes X inactivation. | D'Esposito, M., et al. 1996. Nat Genet. 13: 227-9. PMID: 8640232
- A novel tetanus neurotoxin-insensitive vesicle-associated membrane protein in SNARE complexes of the apical plasma membrane of epithelial cells. | Galli, T., et al. 1998. Mol Biol Cell. 9: 1437-48. PMID: 9614185
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TI-VAMP Antibody (E-12) | sc-166394 | 200 µg/ml | $322.00 | |||
TI-VAMP Antibody (E-12): m-IgGκ BP-HRP Bundle | sc-521588 | 200 µg Ab, 40 µg BP | $361.00 | |||
TI-VAMP Antibody (E-12): m-IgG2b BP-HRP Bundle | sc-548934 | 200 µg Ab; 10 µg BP | $361.00 | |||
TI-VAMP Antibody (E-12) AC | sc-166394 AC | 500 µg/ml, 25% agarose | $424.00 | |||
TI-VAMP Antibody (E-12) HRP | sc-166394 HRP | 200 µg/ml | $322.00 | |||
TI-VAMP Antibody (E-12) FITC | sc-166394 FITC | 200 µg/ml | $336.00 | |||
TI-VAMP Antibody (E-12) PE | sc-166394 PE | 200 µg/ml | $349.00 | |||
TI-VAMP Antibody (E-12) Alexa Fluor® 488 | sc-166394 AF488 | 200 µg/ml | $364.00 | |||
TI-VAMP Antibody (E-12) Alexa Fluor® 546 | sc-166394 AF546 | 200 µg/ml | $364.00 | |||
TI-VAMP Antibody (E-12) Alexa Fluor® 594 | sc-166394 AF594 | 200 µg/ml | $364.00 | |||
TI-VAMP Antibody (E-12) Alexa Fluor® 647 | sc-166394 AF647 | 200 µg/ml | $364.00 | |||
TI-VAMP Antibody (E-12) Alexa Fluor® 680 | sc-166394 AF680 | 200 µg/ml | $364.00 | |||
TI-VAMP Antibody (E-12) Alexa Fluor® 790 | sc-166394 AF790 | 200 µg/ml | $364.00 |